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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 28 — Dec. 31, 2012
  • pp: 29378–29385

Molecular beacons immobilized within suspended core optical fiber for specific DNA detection

Linh Viet Nguyen, Stephen C. Warren-Smith, Alan Cooper, and Tanya M. Monro  »View Author Affiliations


Optics Express, Vol. 20, Issue 28, pp. 29378-29385 (2012)
http://dx.doi.org/10.1364/OE.20.029378


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Abstract

We propose and experimentally demonstrate a new class of sensor for specific DNA sequences based on molecular beacons (MB) immobilized on the internal surfaces of suspended core optical fibers (SCF). MBs, a type of hairpin structured DNA probe, are attached on the surface of the SCF core using a fuzzy nanoassembly process used in conjunction with a biotin-streptavidin-biotin surface attachment strategy. The proposed DNA sensor detects complementary DNA sequences (cDNA) while discriminating sequences differing from the target by just one base. This enables the detection of DNA in unprecedentedly small sample volumes (nL scale) and is, to the best of our knowledge, the first specific DNA detection using a DNA probe immobilized within a microstructured optical fiber.

© 2012 OSA

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(060.4005) Fiber optics and optical communications : Microstructured fibers

ToC Category:
Fiber Optics and Optical Communications

History
Original Manuscript: November 1, 2012
Revised Manuscript: December 3, 2012
Manuscript Accepted: December 3, 2012
Published: December 18, 2012

Virtual Issues
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Linh Viet Nguyen, Stephen C. Warren-Smith, Alan Cooper, and Tanya M. Monro, "Molecular beacons immobilized within suspended core optical fiber for specific DNA detection," Opt. Express 20, 29378-29385 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-28-29378


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