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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 3 — Jan. 30, 2012
  • pp: 2081–2095

Wavelet analysis for single molecule localization microscopy

I. Izeddin, J. Boulanger, V. Racine, C.G. Specht, A. Kechkar, D. Nair, A. Triller, D. Choquet, M. Dahan, and J.B. Sibarita  »View Author Affiliations

Optics Express, Vol. 20, Issue 3, pp. 2081-2095 (2012)

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Localization of single molecules in microscopy images is a key step in quantitative single particle data analysis. Among them, single molecule based super-resolution optical microscopy techniques require high localization accuracy as well as computation of large data sets in the order of 105 single molecule detections to reconstruct a single image. We hereby present an algorithm based on image wavelet segmentation and single particle centroid determination, and compare its performance with the commonly used Gaussian fitting of the point spread function. We performed realistic simulations at different signal-to-noise ratios and particle densities and show that the calculation time using the wavelet approach can be more than one order of magnitude faster than that of Gaussian fitting without a significant degradation of the localization accuracy, from 1 nm to 4 nm in our range of study. We propose a simulation-based estimate of the resolution of an experimental single molecule acquisition.

© 2012 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(100.7410) Image processing : Wavelets
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Image Processing

Original Manuscript: October 3, 2011
Revised Manuscript: December 22, 2011
Manuscript Accepted: January 2, 2012
Published: January 17, 2012

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

I. Izeddin, J. Boulanger, V. Racine, C.G. Specht, A. Kechkar, D. Nair, A. Triller, D. Choquet, M. Dahan, and J.B. Sibarita, "Wavelet analysis for single molecule localization microscopy," Opt. Express 20, 2081-2095 (2012)

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