Optics InfoBase > Optics Express > Volume 20 > Issue 5 > Page 4957
|
PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and trackingIgnacio Izeddin, Mohamed El Beheiry, Jordi Andilla, Daniel Ciepielewski, Xavier Darzacq, and Maxime Dahan »View Author Affiliations
Ignacio Izeddin,1,2
Mohamed El Beheiry,1
Jordi Andilla,3,4
Daniel Ciepielewski,5
Xavier Darzacq,2,6
and Maxime Dahan1,*
1Laboratoire Kastler Brossel, CNRS UMR 8552, Département de Physique et Institut de Biologie de l’Ecole Normale Supérieure, Université Pierre et Marie Curie-Paris6, 46 rue d’Ulm 75230 Paris cedex 05, France 2Laboratoire de Génétique Moléculaire, UMR 8541 (ENS, CNRS, UMPC), Ecole Normale Supérieure, 46 rue d’Ulm 75230 Paris cedex 05, France 3Imagine Optic, 18 rue Charles de Gaulle 91400 Orsay, France 4Currently at: ICFO-Institut de Ciencies Fotoniques, Mediterranean Technology Park, 08860 Castelldefels (Barcelona), Spain 5Nikon France, 191 rue du Marché Rollay 94500 Champigny sur Marne, France 6darzacq@biologie.ens.fr *Corresponding author: maxime.dahan@lkb.ens.fr |
Optics Express, Vol. 20, Issue 5, pp. 4957-4967 (2012)
http://dx.doi.org/10.1364/OE.20.004957
View Full Text Article
Enhanced HTML 
Acrobat PDF (2147 KB)
Abstract
We present a novel approach for three-dimensional localization of single molecules using adaptive optics. A 52-actuator deformable mirror is used to both correct aberrations and induce two-dimensional astigmatism in the point-spread-function. The dependence of the z-localization precision on the degree of astigmatism is discussed. We achieve a z-localization precision of 40 nm for fluorescent proteins and 20 nm for fluorescent dyes, over an axial depth of ~800 nm. We illustrate the capabilities of our approach for three-dimensional high-resolution microscopy with super-resolution images of actin filaments in fixed cells and single-molecule tracking of quantum-dot labeled transmembrane proteins in live HeLa cells.
© 2012 OSA
OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(180.6900) Microscopy : Three-dimensional microscopy
(110.1080) Imaging systems : Active or adaptive optics
ToC Category:
Microscopy
History
Original Manuscript: December 22, 2011
Revised Manuscript: February 6, 2012
Manuscript Accepted: February 8, 2012
Published: February 13, 2012
Virtual Issues
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics
Citation
Ignacio Izeddin, Mohamed El Beheiry, Jordi Andilla, Daniel Ciepielewski, Xavier Darzacq, and Maxime Dahan, "PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking," Opt. Express 20, 4957-4967 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-5-4957
Sort: Author | Year | Journal | Reset
References
- S. J. Lord, H.-L. D. Lee, and W. E. Moerner, “Single-molecule spectroscopy and imaging of biomolecules in living cells,” Anal. Chem.82(6), 2192–2203 (2010). [CrossRef] [PubMed]
- M. Born, E. Wolf, A. B. Bhatia, P. C. Clemmow, D. Gabor, A. R. Stokes, A. M. Taylor, P. A. Wayman, and W. L. Wilcock, Principles of optics: Electromagnetic theory of propagation, interference and diffraction of light, 7th ed. (Cambridge University Press, 1999), p. 986.
- F. V. Subach, G. H. Patterson, S. Manley, J. M. Gillette, J. Lippincott-Schwartz, and V. V. Verkhusha, “Photoactivatable mCherry for high-resolution two-color fluorescence microscopy,” Nat. Methods6(2), 153–159 (2009). [CrossRef] [PubMed]
- S. van de Linde, A. Löschberger, T. Klein, M. Heidbreder, S. Wolter, M. Heilemann, and M. Sauer, “Direct stochastic optical reconstruction microscopy with standard fluorescent probes,” Nat. Protoc.6(7), 991–1009 (2011). [CrossRef] [PubMed]
- M. K. Cheezum, W. F. Walker, and W. H. Guilford, “Quantitative comparison of algorithms for tracking single fluorescent particles,” Biophys. J.81(4), 2378–2388 (2001). [CrossRef] [PubMed]
- R. Henriques, M. Lelek, E. F. Fornasiero, F. Valtorta, C. Zimmer, and M. M. Mhlanga, “QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ,” Nat. Methods7(5), 339–340 (2010). [CrossRef] [PubMed]
- S. Wolter, M. Schüttpelz, M. Tscherepanow, S. VAN DE Linde, M. Heilemann, and M. Sauer, “Real-time computation of subdiffraction-resolution fluorescence images,” J. Microsc.237(1), 12–22 (2010). [CrossRef] [PubMed]
- P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, “Online image analysis software for photoactivation localization microscopy,” Nat. Methods6(10), 689–690 (2009). [CrossRef] [PubMed]
- I. Izeddin, J. Boulanger, V. Racine, C. Specht, A. Kechkar, D. Nair, A. Triller, D. Choquet, M. Dahan, and J. Sibarita, “Wavelet analysis for single molecule localization microscopy,” Opt. Express20(3), 2081–2095 (2012). [CrossRef]
- R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J.82(5), 2775–2783 (2002). [CrossRef] [PubMed]
- S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006). [CrossRef] [PubMed]
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006). [CrossRef] [PubMed]
- G. Patterson, M. Davidson, S. Manley, and J. Lippincott-Schwartz, “Superresolution imaging using single-molecule localization,” Annu. Rev. Phys. Chem.61(1), 345–367 (2010). [CrossRef] [PubMed]
- H. P. Kao and A. S. Verkman, “Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position,” Biophys. J.67(3), 1291–1300 (1994). [CrossRef] [PubMed]
- B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science319(5864), 810–813 (2008). [CrossRef] [PubMed]
- M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods5(6), 527–529 (2008). [CrossRef] [PubMed]
- S. Ram, P. Prabhat, J. Chao, E. S. Ward, and R. J. Ober, “High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells,” Biophys. J.95(12), 6025–6043 (2008). [CrossRef] [PubMed]
- S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- J. Beckers, “Adaptive optics for astronomy: Principles, performance, and applications,” Annu. Rev. Astron. Astr. (1993).
- N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods7, 141–147 (2010). [PubMed]
- M. J. Booth, M. Neil, and T. Wilson, “Aberration correction for confocal imaging in refractive‐index‐mismatched media,” J. Microsc.192(2), 90–98 (1998). [CrossRef]
- M. A. Neil, R. Juskaitis, M. J. Booth, T. Wilson, T. Tanaka, and S. Kawata, “Adaptive aberration correction in a two-photon microscope,” J. Microsc.200(2), 105–108 (2000). [CrossRef] [PubMed]
- O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, “Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons,” Opt. Express18(16), 17521–17532 (2010). [CrossRef] [PubMed]
- X. Tao, B. Fernandez, O. Azucena, M. Fu, D. Garcia, Y. Zuo, D. C. Chen, and J. Kubby, “Adaptive optics confocal microscopy using direct wavefront sensing,” Opt. Lett.36(7), 1062–1064 (2011). [CrossRef] [PubMed]
- R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express2(11), 3135–3149 (2011). [CrossRef] [PubMed]
- Z. Kam, P. Kner, D. Agard, and J. W. Sedat, “Modelling the application of adaptive optics to wide-field microscope live imaging,” J. Microsc.226(1), 33–42 (2007). [CrossRef] [PubMed]
- P. Kner, J. W. Sedat, D. A. Agard, and Z. Kam, “High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing,” J. Microsc.237(2), 136–147 (2010). [CrossRef] [PubMed]
- O. Azucena, J. Crest, S. Kotadia, W. Sullivan, X. Tao, M. Reinig, D. Gavel, S. Olivier, and J. Kubby, “Adaptive optics wide-field microscopy using direct wavefront sensing,” Opt. Lett.36(6), 825–827 (2011). [CrossRef] [PubMed]
- S. Forrest, “Genetic algorithms: principles of natural selection applied to computation,” Science261(5123), 872–878 (1993). [CrossRef] [PubMed]
- M. J. Booth, “Wavefront sensorless adaptive optics for large aberrations,” Opt. Lett.32(1), 5–7 (2007). [CrossRef] [PubMed]
- A. Sergé, N. Bertaux, H. Rigneault, and D. Marguet, “Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes,” Nat. Methods5(8), 687–694 (2008). [CrossRef] [PubMed]
- I. Izeddin, C. G. Specht, M. Lelek, X. Darzacq, A. Triller, C. Zimmer, and M. Dahan, “Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe,” PLoS ONE6(1), e15611 (2011). [CrossRef] [PubMed]
- M. Dahan, S. Lévi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller, “Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking,” Science302(5644), 442–445 (2003). [CrossRef] [PubMed]
- F. Pinaud, S. Clarke, A. Sittner, and M. Dahan, “Probing cellular events, one quantum dot at a time,” Nat. Methods7(4), 275–285 (2010). [CrossRef] [PubMed]
- H. Bannai, S. Lévi, C. Schweizer, M. Dahan, and A. Triller, “Imaging the lateral diffusion of membrane molecules with quantum dots,” Nat. Protoc.1(6), 2628–2634 (2007). [CrossRef] [PubMed]
Agard, D.
Agard, D. A.
Andilla, J.
Artigas, D.
Aviles-Espinosa, R.
Azucena, O.
Bannai, H.
Bates, M.
Bennett, B. T.
Bertaux, N.
Betzig, E.
Bewersdorf, J.
Biteen, J. S.
Bonifacino, J. S.
Booth, M. J.
Boulanger, J.
Cao, J.
Chao, J.
Cheezum, M. K.
Chen, D. C.
Choquet, D.
Clarke, S.
Crest, J.
Dahan, M.
Darzacq, X.
Davidson, M.
Davidson, M. W.
Dillon, D.
Fernandez, B.
Fetter, R. D.
Fornasiero, E. F.
Forrest, S.
Fu, M.
Fuchs, J.
Galbraith, C. G.
Galbraith, J. A.
Garcia, D.
Gavel, D.
Gillette, J. M.
Girirajan, T. P. K.
Gould, T. J.
Guilford, W. H.
Hedde, P. N.
Heidbreder, M.
Heilemann, M.
Henriques, R.
Hess, H. F.
Hess, S. T.
Huang, B.
Izeddin, I.
Ji, N.
Juette, M. F.
Juskaitis, R.
Kam, Z.
Kanchanawong, P.
Kao, H. P.
Kawata, S.
Kechkar, A.
Klein, T.
Kner, P.
Kotadia, S.
Kubby, J.
Larson, D. R.
Lee, H.-L. D.
Lelek, M.
Lessard, M. D.
Levecq, X.
Lévi, S.
Lindwasser, O. W.
Lippincott-Schwartz, J.
Liu, N.
Lord, S. J.
Löschberger, A.
Loza-Alvarez, P.
Luccardini, C.
Manley, S.
Marguet, D.
Mason, M. D.
Mhlanga, M. M.
Milkie, D. E.
Mlodzianoski, M. J.
Moerner, W. E.
Nagpure, B. S.
Nair, D.
Neil, M.
Neil, M. A.
Nienhaus, G. U.
Nieto, M.
Ober, R. J.
Olarte, O. E.
Olenych, S.
Olivier, S.
Oswald, F.
Patterson, G.
Patterson, G. H.
Pavani, S. R. P.
Piestun, R.
Pinaud, F.
Porcar-Guezenec, R.
Prabhat, P.
Racine, V.
Ram, S.
Reinig, M.
Rigneault, H.
Riveau, B.
Rostaing, P.
Rust, M. J.
Sauer, M.
Schüttpelz, M.
Schweizer, C.
Sedat, J. W.
Sergé, A.
Shtengel, G.
Sibarita, J.
Sittner, A.
Sougrat, R.
Specht, C.
Specht, C. G.
Subach, F. V.
Sullivan, W.
Tanaka, T.
Tao, X.
Thompson, M. A.
Thompson, R. E.
Triller, A.
Tscherepanow, M.
Twieg, R. J.
Valtorta, F.
van de Linde, S.
Verkhusha, V. V.
Verkman, A. S.
Walker, W. F.
Wang, W.
Ward, E. S.
Waterman, C. M.
Webb, W. W.
Wiedenmann, J.
Wilson, T.
Wolter, S.
Zhuang, X.
Zimmer, C.
Zuo, Y.
- Z. Kam, P. Kner, D. Agard, and J. W. Sedat, “Modelling the application of adaptive optics to wide-field microscope live imaging,” J. Microsc.226(1), 33–42 (2007). [CrossRef] [PubMed]
- P. Kner, J. W. Sedat, D. A. Agard, and Z. Kam, “High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing,” J. Microsc.237(2), 136–147 (2010). [CrossRef] [PubMed]
- R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express2(11), 3135–3149 (2011). [CrossRef] [PubMed]
- R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express2(11), 3135–3149 (2011). [CrossRef] [PubMed]
- R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express2(11), 3135–3149 (2011). [CrossRef] [PubMed]
- X. Tao, B. Fernandez, O. Azucena, M. Fu, D. Garcia, Y. Zuo, D. C. Chen, and J. Kubby, “Adaptive optics confocal microscopy using direct wavefront sensing,” Opt. Lett.36(7), 1062–1064 (2011). [CrossRef] [PubMed]
- O. Azucena, J. Crest, S. Kotadia, W. Sullivan, X. Tao, M. Reinig, D. Gavel, S. Olivier, and J. Kubby, “Adaptive optics wide-field microscopy using direct wavefront sensing,” Opt. Lett.36(6), 825–827 (2011). [CrossRef] [PubMed]
- O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, “Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons,” Opt. Express18(16), 17521–17532 (2010). [CrossRef] [PubMed]
- H. Bannai, S. Lévi, C. Schweizer, M. Dahan, and A. Triller, “Imaging the lateral diffusion of membrane molecules with quantum dots,” Nat. Protoc.1(6), 2628–2634 (2007). [CrossRef] [PubMed]
- B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science319(5864), 810–813 (2008). [CrossRef] [PubMed]
- M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006). [CrossRef] [PubMed]
- M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods5(6), 527–529 (2008). [CrossRef] [PubMed]
- A. Sergé, N. Bertaux, H. Rigneault, and D. Marguet, “Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes,” Nat. Methods5(8), 687–694 (2008). [CrossRef] [PubMed]
- N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods7, 141–147 (2010). [PubMed]
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods5(6), 527–529 (2008). [CrossRef] [PubMed]
- S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- M. J. Booth, “Wavefront sensorless adaptive optics for large aberrations,” Opt. Lett.32(1), 5–7 (2007). [CrossRef] [PubMed]
- M. A. Neil, R. Juskaitis, M. J. Booth, T. Wilson, T. Tanaka, and S. Kawata, “Adaptive aberration correction in a two-photon microscope,” J. Microsc.200(2), 105–108 (2000). [CrossRef] [PubMed]
- M. J. Booth, M. Neil, and T. Wilson, “Aberration correction for confocal imaging in refractive‐index‐mismatched media,” J. Microsc.192(2), 90–98 (1998). [CrossRef]
- S. Ram, P. Prabhat, J. Chao, E. S. Ward, and R. J. Ober, “High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells,” Biophys. J.95(12), 6025–6043 (2008). [CrossRef] [PubMed]
- M. K. Cheezum, W. F. Walker, and W. H. Guilford, “Quantitative comparison of algorithms for tracking single fluorescent particles,” Biophys. J.81(4), 2378–2388 (2001). [CrossRef] [PubMed]
- F. Pinaud, S. Clarke, A. Sittner, and M. Dahan, “Probing cellular events, one quantum dot at a time,” Nat. Methods7(4), 275–285 (2010). [CrossRef] [PubMed]
- O. Azucena, J. Crest, S. Kotadia, W. Sullivan, X. Tao, M. Reinig, D. Gavel, S. Olivier, and J. Kubby, “Adaptive optics wide-field microscopy using direct wavefront sensing,” Opt. Lett.36(6), 825–827 (2011). [CrossRef] [PubMed]
- O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, “Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons,” Opt. Express18(16), 17521–17532 (2010). [CrossRef] [PubMed]
- I. Izeddin, J. Boulanger, V. Racine, C. Specht, A. Kechkar, D. Nair, A. Triller, D. Choquet, M. Dahan, and J. Sibarita, “Wavelet analysis for single molecule localization microscopy,” Opt. Express20(3), 2081–2095 (2012). [CrossRef]
- I. Izeddin, C. G. Specht, M. Lelek, X. Darzacq, A. Triller, C. Zimmer, and M. Dahan, “Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe,” PLoS ONE6(1), e15611 (2011). [CrossRef] [PubMed]
- F. Pinaud, S. Clarke, A. Sittner, and M. Dahan, “Probing cellular events, one quantum dot at a time,” Nat. Methods7(4), 275–285 (2010). [CrossRef] [PubMed]
- H. Bannai, S. Lévi, C. Schweizer, M. Dahan, and A. Triller, “Imaging the lateral diffusion of membrane molecules with quantum dots,” Nat. Protoc.1(6), 2628–2634 (2007). [CrossRef] [PubMed]
- M. Dahan, S. Lévi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller, “Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking,” Science302(5644), 442–445 (2003). [CrossRef] [PubMed]
- I. Izeddin, C. G. Specht, M. Lelek, X. Darzacq, A. Triller, C. Zimmer, and M. Dahan, “Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe,” PLoS ONE6(1), e15611 (2011). [CrossRef] [PubMed]
- G. Patterson, M. Davidson, S. Manley, and J. Lippincott-Schwartz, “Superresolution imaging using single-molecule localization,” Annu. Rev. Phys. Chem.61(1), 345–367 (2010). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- R. Henriques, M. Lelek, E. F. Fornasiero, F. Valtorta, C. Zimmer, and M. M. Mhlanga, “QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ,” Nat. Methods7(5), 339–340 (2010). [CrossRef] [PubMed]
- S. Forrest, “Genetic algorithms: principles of natural selection applied to computation,” Science261(5123), 872–878 (1993). [CrossRef] [PubMed]
- P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, “Online image analysis software for photoactivation localization microscopy,” Nat. Methods6(10), 689–690 (2009). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- O. Azucena, J. Crest, S. Kotadia, W. Sullivan, X. Tao, M. Reinig, D. Gavel, S. Olivier, and J. Kubby, “Adaptive optics wide-field microscopy using direct wavefront sensing,” Opt. Lett.36(6), 825–827 (2011). [CrossRef] [PubMed]
- O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, “Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons,” Opt. Express18(16), 17521–17532 (2010). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- F. V. Subach, G. H. Patterson, S. Manley, J. M. Gillette, J. Lippincott-Schwartz, and V. V. Verkhusha, “Photoactivatable mCherry for high-resolution two-color fluorescence microscopy,” Nat. Methods6(2), 153–159 (2009). [CrossRef] [PubMed]
- S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006). [CrossRef] [PubMed]
- M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods5(6), 527–529 (2008). [CrossRef] [PubMed]
- M. K. Cheezum, W. F. Walker, and W. H. Guilford, “Quantitative comparison of algorithms for tracking single fluorescent particles,” Biophys. J.81(4), 2378–2388 (2001). [CrossRef] [PubMed]
- P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, “Online image analysis software for photoactivation localization microscopy,” Nat. Methods6(10), 689–690 (2009). [CrossRef] [PubMed]
- S. van de Linde, A. Löschberger, T. Klein, M. Heidbreder, S. Wolter, M. Heilemann, and M. Sauer, “Direct stochastic optical reconstruction microscopy with standard fluorescent probes,” Nat. Protoc.6(7), 991–1009 (2011). [CrossRef] [PubMed]
- S. van de Linde, A. Löschberger, T. Klein, M. Heidbreder, S. Wolter, M. Heilemann, and M. Sauer, “Direct stochastic optical reconstruction microscopy with standard fluorescent probes,” Nat. Protoc.6(7), 991–1009 (2011). [CrossRef] [PubMed]
- S. Wolter, M. Schüttpelz, M. Tscherepanow, S. VAN DE Linde, M. Heilemann, and M. Sauer, “Real-time computation of subdiffraction-resolution fluorescence images,” J. Microsc.237(1), 12–22 (2010). [CrossRef] [PubMed]
- R. Henriques, M. Lelek, E. F. Fornasiero, F. Valtorta, C. Zimmer, and M. M. Mhlanga, “QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ,” Nat. Methods7(5), 339–340 (2010). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods5(6), 527–529 (2008). [CrossRef] [PubMed]
- S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006). [CrossRef] [PubMed]
- B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science319(5864), 810–813 (2008). [CrossRef] [PubMed]
- I. Izeddin, J. Boulanger, V. Racine, C. Specht, A. Kechkar, D. Nair, A. Triller, D. Choquet, M. Dahan, and J. Sibarita, “Wavelet analysis for single molecule localization microscopy,” Opt. Express20(3), 2081–2095 (2012). [CrossRef]
- I. Izeddin, C. G. Specht, M. Lelek, X. Darzacq, A. Triller, C. Zimmer, and M. Dahan, “Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe,” PLoS ONE6(1), e15611 (2011). [CrossRef] [PubMed]
- N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods7, 141–147 (2010). [PubMed]
- M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods5(6), 527–529 (2008). [CrossRef] [PubMed]
- M. A. Neil, R. Juskaitis, M. J. Booth, T. Wilson, T. Tanaka, and S. Kawata, “Adaptive aberration correction in a two-photon microscope,” J. Microsc.200(2), 105–108 (2000). [CrossRef] [PubMed]
- P. Kner, J. W. Sedat, D. A. Agard, and Z. Kam, “High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing,” J. Microsc.237(2), 136–147 (2010). [CrossRef] [PubMed]
- Z. Kam, P. Kner, D. Agard, and J. W. Sedat, “Modelling the application of adaptive optics to wide-field microscope live imaging,” J. Microsc.226(1), 33–42 (2007). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- H. P. Kao and A. S. Verkman, “Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position,” Biophys. J.67(3), 1291–1300 (1994). [CrossRef] [PubMed]
- M. A. Neil, R. Juskaitis, M. J. Booth, T. Wilson, T. Tanaka, and S. Kawata, “Adaptive aberration correction in a two-photon microscope,” J. Microsc.200(2), 105–108 (2000). [CrossRef] [PubMed]
- S. van de Linde, A. Löschberger, T. Klein, M. Heidbreder, S. Wolter, M. Heilemann, and M. Sauer, “Direct stochastic optical reconstruction microscopy with standard fluorescent probes,” Nat. Protoc.6(7), 991–1009 (2011). [CrossRef] [PubMed]
- O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, “Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons,” Opt. Express18(16), 17521–17532 (2010). [CrossRef] [PubMed]
- P. Kner, J. W. Sedat, D. A. Agard, and Z. Kam, “High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing,” J. Microsc.237(2), 136–147 (2010). [CrossRef] [PubMed]
- Z. Kam, P. Kner, D. Agard, and J. W. Sedat, “Modelling the application of adaptive optics to wide-field microscope live imaging,” J. Microsc.226(1), 33–42 (2007). [CrossRef] [PubMed]
- O. Azucena, J. Crest, S. Kotadia, W. Sullivan, X. Tao, M. Reinig, D. Gavel, S. Olivier, and J. Kubby, “Adaptive optics wide-field microscopy using direct wavefront sensing,” Opt. Lett.36(6), 825–827 (2011). [CrossRef] [PubMed]
- X. Tao, B. Fernandez, O. Azucena, M. Fu, D. Garcia, Y. Zuo, D. C. Chen, and J. Kubby, “Adaptive optics confocal microscopy using direct wavefront sensing,” Opt. Lett.36(7), 1062–1064 (2011). [CrossRef] [PubMed]
- O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, “Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons,” Opt. Express18(16), 17521–17532 (2010). [CrossRef] [PubMed]
- R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J.82(5), 2775–2783 (2002). [CrossRef] [PubMed]
- S. J. Lord, H.-L. D. Lee, and W. E. Moerner, “Single-molecule spectroscopy and imaging of biomolecules in living cells,” Anal. Chem.82(6), 2192–2203 (2010). [CrossRef] [PubMed]
- I. Izeddin, C. G. Specht, M. Lelek, X. Darzacq, A. Triller, C. Zimmer, and M. Dahan, “Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe,” PLoS ONE6(1), e15611 (2011). [CrossRef] [PubMed]
- R. Henriques, M. Lelek, E. F. Fornasiero, F. Valtorta, C. Zimmer, and M. M. Mhlanga, “QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ,” Nat. Methods7(5), 339–340 (2010). [CrossRef] [PubMed]
- M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods5(6), 527–529 (2008). [CrossRef] [PubMed]
- R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express2(11), 3135–3149 (2011). [CrossRef] [PubMed]
- H. Bannai, S. Lévi, C. Schweizer, M. Dahan, and A. Triller, “Imaging the lateral diffusion of membrane molecules with quantum dots,” Nat. Protoc.1(6), 2628–2634 (2007). [CrossRef] [PubMed]
- M. Dahan, S. Lévi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller, “Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking,” Science302(5644), 442–445 (2003). [CrossRef] [PubMed]
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- G. Patterson, M. Davidson, S. Manley, and J. Lippincott-Schwartz, “Superresolution imaging using single-molecule localization,” Annu. Rev. Phys. Chem.61(1), 345–367 (2010). [CrossRef] [PubMed]
- F. V. Subach, G. H. Patterson, S. Manley, J. M. Gillette, J. Lippincott-Schwartz, and V. V. Verkhusha, “Photoactivatable mCherry for high-resolution two-color fluorescence microscopy,” Nat. Methods6(2), 153–159 (2009). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
- S. J. Lord, H.-L. D. Lee, and W. E. Moerner, “Single-molecule spectroscopy and imaging of biomolecules in living cells,” Anal. Chem.82(6), 2192–2203 (2010). [CrossRef] [PubMed]
- S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
- S. van de Linde, A. Löschberger, T. Klein, M. Heidbreder, S. Wolter, M. Heilemann, and M. Sauer, “Direct stochastic optical reconstruction microscopy with standard fluorescent probes,” Nat. Protoc.6(7), 991–1009 (2011). [CrossRef] [PubMed]
- R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express2(11), 3135–3149 (2011). [CrossRef] [PubMed]
- M. Dahan, S. Lévi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller, “Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking,” Science302(5644), 442–445 (2003). [CrossRef] [PubMed]
- G. Patterson, M. Davidson, S. Manley, and J. Lippincott-Schwartz, “Superresolution imaging using single-molecule localization,” Annu. Rev. Phys. Chem.61(1), 345–367 (2010). [CrossRef] [PubMed]
- F. V. Subach, G. H. Patterson, S. Manley, J. M. Gillette, J. Lippincott-Schwartz, and V. V. Verkhusha, “Photoactivatable mCherry for high-resolution two-color fluorescence microscopy,” Nat. Methods6(2), 153–159 (2009). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- A. Sergé, N. Bertaux, H. Rigneault, and D. Marguet, “Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes,” Nat. Methods5(8), 687–694 (2008). [CrossRef] [PubMed]
- S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006). [CrossRef] [PubMed]
- R. Henriques, M. Lelek, E. F. Fornasiero, F. Valtorta, C. Zimmer, and M. M. Mhlanga, “QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ,” Nat. Methods7(5), 339–340 (2010). [CrossRef] [PubMed]
- N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods7, 141–147 (2010). [PubMed]
- M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods5(6), 527–529 (2008). [CrossRef] [PubMed]
- S. J. Lord, H.-L. D. Lee, and W. E. Moerner, “Single-molecule spectroscopy and imaging of biomolecules in living cells,” Anal. Chem.82(6), 2192–2203 (2010). [CrossRef] [PubMed]
- S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
- M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods5(6), 527–529 (2008). [CrossRef] [PubMed]
- M. J. Booth, M. Neil, and T. Wilson, “Aberration correction for confocal imaging in refractive‐index‐mismatched media,” J. Microsc.192(2), 90–98 (1998). [CrossRef]
- M. A. Neil, R. Juskaitis, M. J. Booth, T. Wilson, T. Tanaka, and S. Kawata, “Adaptive aberration correction in a two-photon microscope,” J. Microsc.200(2), 105–108 (2000). [CrossRef] [PubMed]
- P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, “Online image analysis software for photoactivation localization microscopy,” Nat. Methods6(10), 689–690 (2009). [CrossRef] [PubMed]
- R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express2(11), 3135–3149 (2011). [CrossRef] [PubMed]
- S. Ram, P. Prabhat, J. Chao, E. S. Ward, and R. J. Ober, “High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells,” Biophys. J.95(12), 6025–6043 (2008). [CrossRef] [PubMed]
- R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express2(11), 3135–3149 (2011). [CrossRef] [PubMed]
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- O. Azucena, J. Crest, S. Kotadia, W. Sullivan, X. Tao, M. Reinig, D. Gavel, S. Olivier, and J. Kubby, “Adaptive optics wide-field microscopy using direct wavefront sensing,” Opt. Lett.36(6), 825–827 (2011). [CrossRef] [PubMed]
- O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, “Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons,” Opt. Express18(16), 17521–17532 (2010). [CrossRef] [PubMed]
- P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, “Online image analysis software for photoactivation localization microscopy,” Nat. Methods6(10), 689–690 (2009). [CrossRef] [PubMed]
- G. Patterson, M. Davidson, S. Manley, and J. Lippincott-Schwartz, “Superresolution imaging using single-molecule localization,” Annu. Rev. Phys. Chem.61(1), 345–367 (2010). [CrossRef] [PubMed]
- F. V. Subach, G. H. Patterson, S. Manley, J. M. Gillette, J. Lippincott-Schwartz, and V. V. Verkhusha, “Photoactivatable mCherry for high-resolution two-color fluorescence microscopy,” Nat. Methods6(2), 153–159 (2009). [CrossRef] [PubMed]
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
- S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
- F. Pinaud, S. Clarke, A. Sittner, and M. Dahan, “Probing cellular events, one quantum dot at a time,” Nat. Methods7(4), 275–285 (2010). [CrossRef] [PubMed]
- R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express2(11), 3135–3149 (2011). [CrossRef] [PubMed]
- S. Ram, P. Prabhat, J. Chao, E. S. Ward, and R. J. Ober, “High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells,” Biophys. J.95(12), 6025–6043 (2008). [CrossRef] [PubMed]
- S. Ram, P. Prabhat, J. Chao, E. S. Ward, and R. J. Ober, “High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells,” Biophys. J.95(12), 6025–6043 (2008). [CrossRef] [PubMed]
- A. Sergé, N. Bertaux, H. Rigneault, and D. Marguet, “Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes,” Nat. Methods5(8), 687–694 (2008). [CrossRef] [PubMed]
- M. Dahan, S. Lévi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller, “Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking,” Science302(5644), 442–445 (2003). [CrossRef] [PubMed]
- M. Dahan, S. Lévi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller, “Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking,” Science302(5644), 442–445 (2003). [CrossRef] [PubMed]
- M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006). [CrossRef] [PubMed]
- S. van de Linde, A. Löschberger, T. Klein, M. Heidbreder, S. Wolter, M. Heilemann, and M. Sauer, “Direct stochastic optical reconstruction microscopy with standard fluorescent probes,” Nat. Protoc.6(7), 991–1009 (2011). [CrossRef] [PubMed]
- S. Wolter, M. Schüttpelz, M. Tscherepanow, S. VAN DE Linde, M. Heilemann, and M. Sauer, “Real-time computation of subdiffraction-resolution fluorescence images,” J. Microsc.237(1), 12–22 (2010). [CrossRef] [PubMed]
- S. Wolter, M. Schüttpelz, M. Tscherepanow, S. VAN DE Linde, M. Heilemann, and M. Sauer, “Real-time computation of subdiffraction-resolution fluorescence images,” J. Microsc.237(1), 12–22 (2010). [CrossRef] [PubMed]
- H. Bannai, S. Lévi, C. Schweizer, M. Dahan, and A. Triller, “Imaging the lateral diffusion of membrane molecules with quantum dots,” Nat. Protoc.1(6), 2628–2634 (2007). [CrossRef] [PubMed]
- P. Kner, J. W. Sedat, D. A. Agard, and Z. Kam, “High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing,” J. Microsc.237(2), 136–147 (2010). [CrossRef] [PubMed]
- Z. Kam, P. Kner, D. Agard, and J. W. Sedat, “Modelling the application of adaptive optics to wide-field microscope live imaging,” J. Microsc.226(1), 33–42 (2007). [CrossRef] [PubMed]
- A. Sergé, N. Bertaux, H. Rigneault, and D. Marguet, “Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes,” Nat. Methods5(8), 687–694 (2008). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- F. Pinaud, S. Clarke, A. Sittner, and M. Dahan, “Probing cellular events, one quantum dot at a time,” Nat. Methods7(4), 275–285 (2010). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- I. Izeddin, C. G. Specht, M. Lelek, X. Darzacq, A. Triller, C. Zimmer, and M. Dahan, “Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe,” PLoS ONE6(1), e15611 (2011). [CrossRef] [PubMed]
- F. V. Subach, G. H. Patterson, S. Manley, J. M. Gillette, J. Lippincott-Schwartz, and V. V. Verkhusha, “Photoactivatable mCherry for high-resolution two-color fluorescence microscopy,” Nat. Methods6(2), 153–159 (2009). [CrossRef] [PubMed]
- O. Azucena, J. Crest, S. Kotadia, W. Sullivan, X. Tao, M. Reinig, D. Gavel, S. Olivier, and J. Kubby, “Adaptive optics wide-field microscopy using direct wavefront sensing,” Opt. Lett.36(6), 825–827 (2011). [CrossRef] [PubMed]
- O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, “Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons,” Opt. Express18(16), 17521–17532 (2010). [CrossRef] [PubMed]
- M. A. Neil, R. Juskaitis, M. J. Booth, T. Wilson, T. Tanaka, and S. Kawata, “Adaptive aberration correction in a two-photon microscope,” J. Microsc.200(2), 105–108 (2000). [CrossRef] [PubMed]
- X. Tao, B. Fernandez, O. Azucena, M. Fu, D. Garcia, Y. Zuo, D. C. Chen, and J. Kubby, “Adaptive optics confocal microscopy using direct wavefront sensing,” Opt. Lett.36(7), 1062–1064 (2011). [CrossRef] [PubMed]
- O. Azucena, J. Crest, S. Kotadia, W. Sullivan, X. Tao, M. Reinig, D. Gavel, S. Olivier, and J. Kubby, “Adaptive optics wide-field microscopy using direct wavefront sensing,” Opt. Lett.36(6), 825–827 (2011). [CrossRef] [PubMed]
- S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
- R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J.82(5), 2775–2783 (2002). [CrossRef] [PubMed]
- I. Izeddin, J. Boulanger, V. Racine, C. Specht, A. Kechkar, D. Nair, A. Triller, D. Choquet, M. Dahan, and J. Sibarita, “Wavelet analysis for single molecule localization microscopy,” Opt. Express20(3), 2081–2095 (2012). [CrossRef]
- I. Izeddin, C. G. Specht, M. Lelek, X. Darzacq, A. Triller, C. Zimmer, and M. Dahan, “Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe,” PLoS ONE6(1), e15611 (2011). [CrossRef] [PubMed]
- H. Bannai, S. Lévi, C. Schweizer, M. Dahan, and A. Triller, “Imaging the lateral diffusion of membrane molecules with quantum dots,” Nat. Protoc.1(6), 2628–2634 (2007). [CrossRef] [PubMed]
- M. Dahan, S. Lévi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller, “Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking,” Science302(5644), 442–445 (2003). [CrossRef] [PubMed]
- S. Wolter, M. Schüttpelz, M. Tscherepanow, S. VAN DE Linde, M. Heilemann, and M. Sauer, “Real-time computation of subdiffraction-resolution fluorescence images,” J. Microsc.237(1), 12–22 (2010). [CrossRef] [PubMed]
- S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
- R. Henriques, M. Lelek, E. F. Fornasiero, F. Valtorta, C. Zimmer, and M. M. Mhlanga, “QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ,” Nat. Methods7(5), 339–340 (2010). [CrossRef] [PubMed]
- S. van de Linde, A. Löschberger, T. Klein, M. Heidbreder, S. Wolter, M. Heilemann, and M. Sauer, “Direct stochastic optical reconstruction microscopy with standard fluorescent probes,” Nat. Protoc.6(7), 991–1009 (2011). [CrossRef] [PubMed]
- S. Wolter, M. Schüttpelz, M. Tscherepanow, S. VAN DE Linde, M. Heilemann, and M. Sauer, “Real-time computation of subdiffraction-resolution fluorescence images,” J. Microsc.237(1), 12–22 (2010). [CrossRef] [PubMed]
- F. V. Subach, G. H. Patterson, S. Manley, J. M. Gillette, J. Lippincott-Schwartz, and V. V. Verkhusha, “Photoactivatable mCherry for high-resolution two-color fluorescence microscopy,” Nat. Methods6(2), 153–159 (2009). [CrossRef] [PubMed]
- H. P. Kao and A. S. Verkman, “Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position,” Biophys. J.67(3), 1291–1300 (1994). [CrossRef] [PubMed]
- M. K. Cheezum, W. F. Walker, and W. H. Guilford, “Quantitative comparison of algorithms for tracking single fluorescent particles,” Biophys. J.81(4), 2378–2388 (2001). [CrossRef] [PubMed]
- B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science319(5864), 810–813 (2008). [CrossRef] [PubMed]
- S. Ram, P. Prabhat, J. Chao, E. S. Ward, and R. J. Ober, “High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells,” Biophys. J.95(12), 6025–6043 (2008). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J.82(5), 2775–2783 (2002). [CrossRef] [PubMed]
- P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, “Online image analysis software for photoactivation localization microscopy,” Nat. Methods6(10), 689–690 (2009). [CrossRef] [PubMed]
- M. A. Neil, R. Juskaitis, M. J. Booth, T. Wilson, T. Tanaka, and S. Kawata, “Adaptive aberration correction in a two-photon microscope,” J. Microsc.200(2), 105–108 (2000). [CrossRef] [PubMed]
- M. J. Booth, M. Neil, and T. Wilson, “Aberration correction for confocal imaging in refractive‐index‐mismatched media,” J. Microsc.192(2), 90–98 (1998). [CrossRef]
- S. van de Linde, A. Löschberger, T. Klein, M. Heidbreder, S. Wolter, M. Heilemann, and M. Sauer, “Direct stochastic optical reconstruction microscopy with standard fluorescent probes,” Nat. Protoc.6(7), 991–1009 (2011). [CrossRef] [PubMed]
- S. Wolter, M. Schüttpelz, M. Tscherepanow, S. VAN DE Linde, M. Heilemann, and M. Sauer, “Real-time computation of subdiffraction-resolution fluorescence images,” J. Microsc.237(1), 12–22 (2010). [CrossRef] [PubMed]
- B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science319(5864), 810–813 (2008). [CrossRef] [PubMed]
- M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006). [CrossRef] [PubMed]
- I. Izeddin, C. G. Specht, M. Lelek, X. Darzacq, A. Triller, C. Zimmer, and M. Dahan, “Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe,” PLoS ONE6(1), e15611 (2011). [CrossRef] [PubMed]
- R. Henriques, M. Lelek, E. F. Fornasiero, F. Valtorta, C. Zimmer, and M. M. Mhlanga, “QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ,” Nat. Methods7(5), 339–340 (2010). [CrossRef] [PubMed]
Anal. Chem.
- S. J. Lord, H.-L. D. Lee, and W. E. Moerner, “Single-molecule spectroscopy and imaging of biomolecules in living cells,” Anal. Chem.82(6), 2192–2203 (2010). [CrossRef] [PubMed]
Annu. Rev. Phys. Chem.
- G. Patterson, M. Davidson, S. Manley, and J. Lippincott-Schwartz, “Superresolution imaging using single-molecule localization,” Annu. Rev. Phys. Chem.61(1), 345–367 (2010). [CrossRef] [PubMed]
Biomed. Opt. Express
- R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express2(11), 3135–3149 (2011). [CrossRef] [PubMed]
Biophys. J.
- H. P. Kao and A. S. Verkman, “Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position,” Biophys. J.67(3), 1291–1300 (1994). [CrossRef] [PubMed]
- R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J.82(5), 2775–2783 (2002). [CrossRef] [PubMed]
- S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006). [CrossRef] [PubMed]
- S. Ram, P. Prabhat, J. Chao, E. S. Ward, and R. J. Ober, “High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells,” Biophys. J.95(12), 6025–6043 (2008). [CrossRef] [PubMed]
- M. K. Cheezum, W. F. Walker, and W. H. Guilford, “Quantitative comparison of algorithms for tracking single fluorescent particles,” Biophys. J.81(4), 2378–2388 (2001). [CrossRef] [PubMed]
J. Microsc.
- S. Wolter, M. Schüttpelz, M. Tscherepanow, S. VAN DE Linde, M. Heilemann, and M. Sauer, “Real-time computation of subdiffraction-resolution fluorescence images,” J. Microsc.237(1), 12–22 (2010). [CrossRef] [PubMed]
- Z. Kam, P. Kner, D. Agard, and J. W. Sedat, “Modelling the application of adaptive optics to wide-field microscope live imaging,” J. Microsc.226(1), 33–42 (2007). [CrossRef] [PubMed]
- P. Kner, J. W. Sedat, D. A. Agard, and Z. Kam, “High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing,” J. Microsc.237(2), 136–147 (2010). [CrossRef] [PubMed]
- M. J. Booth, M. Neil, and T. Wilson, “Aberration correction for confocal imaging in refractive‐index‐mismatched media,” J. Microsc.192(2), 90–98 (1998). [CrossRef]
- M. A. Neil, R. Juskaitis, M. J. Booth, T. Wilson, T. Tanaka, and S. Kawata, “Adaptive aberration correction in a two-photon microscope,” J. Microsc.200(2), 105–108 (2000). [CrossRef] [PubMed]
Nat. Methods
- M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006). [CrossRef] [PubMed]
- M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods5(6), 527–529 (2008). [CrossRef] [PubMed]
- N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods7, 141–147 (2010). [PubMed]
- A. Sergé, N. Bertaux, H. Rigneault, and D. Marguet, “Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes,” Nat. Methods5(8), 687–694 (2008). [CrossRef] [PubMed]
- F. Pinaud, S. Clarke, A. Sittner, and M. Dahan, “Probing cellular events, one quantum dot at a time,” Nat. Methods7(4), 275–285 (2010). [CrossRef] [PubMed]
- P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, “Online image analysis software for photoactivation localization microscopy,” Nat. Methods6(10), 689–690 (2009). [CrossRef] [PubMed]
- R. Henriques, M. Lelek, E. F. Fornasiero, F. Valtorta, C. Zimmer, and M. M. Mhlanga, “QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ,” Nat. Methods7(5), 339–340 (2010). [CrossRef] [PubMed]
- F. V. Subach, G. H. Patterson, S. Manley, J. M. Gillette, J. Lippincott-Schwartz, and V. V. Verkhusha, “Photoactivatable mCherry for high-resolution two-color fluorescence microscopy,” Nat. Methods6(2), 153–159 (2009). [CrossRef] [PubMed]
Nat. Protoc.
- S. van de Linde, A. Löschberger, T. Klein, M. Heidbreder, S. Wolter, M. Heilemann, and M. Sauer, “Direct stochastic optical reconstruction microscopy with standard fluorescent probes,” Nat. Protoc.6(7), 991–1009 (2011). [CrossRef] [PubMed]
- H. Bannai, S. Lévi, C. Schweizer, M. Dahan, and A. Triller, “Imaging the lateral diffusion of membrane molecules with quantum dots,” Nat. Protoc.1(6), 2628–2634 (2007). [CrossRef] [PubMed]
Opt. Express
- O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, “Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons,” Opt. Express18(16), 17521–17532 (2010). [CrossRef] [PubMed]
- I. Izeddin, J. Boulanger, V. Racine, C. Specht, A. Kechkar, D. Nair, A. Triller, D. Choquet, M. Dahan, and J. Sibarita, “Wavelet analysis for single molecule localization microscopy,” Opt. Express20(3), 2081–2095 (2012). [CrossRef]
Opt. Lett.
- X. Tao, B. Fernandez, O. Azucena, M. Fu, D. Garcia, Y. Zuo, D. C. Chen, and J. Kubby, “Adaptive optics confocal microscopy using direct wavefront sensing,” Opt. Lett.36(7), 1062–1064 (2011). [CrossRef] [PubMed]
- O. Azucena, J. Crest, S. Kotadia, W. Sullivan, X. Tao, M. Reinig, D. Gavel, S. Olivier, and J. Kubby, “Adaptive optics wide-field microscopy using direct wavefront sensing,” Opt. Lett.36(6), 825–827 (2011). [CrossRef] [PubMed]
- M. J. Booth, “Wavefront sensorless adaptive optics for large aberrations,” Opt. Lett.32(1), 5–7 (2007). [CrossRef] [PubMed]
PLoS ONE
- I. Izeddin, C. G. Specht, M. Lelek, X. Darzacq, A. Triller, C. Zimmer, and M. Dahan, “Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe,” PLoS ONE6(1), e15611 (2011). [CrossRef] [PubMed]
Proc. Natl. Acad. Sci. U.S.A.
- S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
Science
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science319(5864), 810–813 (2008). [CrossRef] [PubMed]
- M. Dahan, S. Lévi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller, “Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking,” Science302(5644), 442–445 (2003). [CrossRef] [PubMed]
- S. Forrest, “Genetic algorithms: principles of natural selection applied to computation,” Science261(5123), 872–878 (1993). [CrossRef] [PubMed]
Other
- J. Beckers, “Adaptive optics for astronomy: Principles, performance, and applications,” Annu. Rev. Astron. Astr. (1993).
- M. Born, E. Wolf, A. B. Bhatia, P. C. Clemmow, D. Gabor, A. R. Stokes, A. M. Taylor, P. A. Wayman, and W. L. Wilcock, Principles of optics: Electromagnetic theory of propagation, interference and diffraction of light, 7th ed. (Cambridge University Press, 1999), p. 986.
2012, Izeddin, Opt. Express
- S. van de Linde, A. Löschberger, T. Klein, M. Heidbreder, S. Wolter, M. Heilemann, and M. Sauer, “Direct stochastic optical reconstruction microscopy with standard fluorescent probes,” Nat. Protoc.6(7), 991–1009 (2011). [CrossRef] [PubMed]
- R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express2(11), 3135–3149 (2011). [CrossRef] [PubMed]
- I. Izeddin, C. G. Specht, M. Lelek, X. Darzacq, A. Triller, C. Zimmer, and M. Dahan, “Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe,” PLoS ONE6(1), e15611 (2011). [CrossRef] [PubMed]
- P. Kner, J. W. Sedat, D. A. Agard, and Z. Kam, “High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing,” J. Microsc.237(2), 136–147 (2010). [CrossRef] [PubMed]
- F. Pinaud, S. Clarke, A. Sittner, and M. Dahan, “Probing cellular events, one quantum dot at a time,” Nat. Methods7(4), 275–285 (2010). [CrossRef] [PubMed]
- N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods7, 141–147 (2010). [PubMed]
- S. J. Lord, H.-L. D. Lee, and W. E. Moerner, “Single-molecule spectroscopy and imaging of biomolecules in living cells,” Anal. Chem.82(6), 2192–2203 (2010). [CrossRef] [PubMed]
- R. Henriques, M. Lelek, E. F. Fornasiero, F. Valtorta, C. Zimmer, and M. M. Mhlanga, “QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ,” Nat. Methods7(5), 339–340 (2010). [CrossRef] [PubMed]
- S. Wolter, M. Schüttpelz, M. Tscherepanow, S. VAN DE Linde, M. Heilemann, and M. Sauer, “Real-time computation of subdiffraction-resolution fluorescence images,” J. Microsc.237(1), 12–22 (2010). [CrossRef] [PubMed]
- G. Patterson, M. Davidson, S. Manley, and J. Lippincott-Schwartz, “Superresolution imaging using single-molecule localization,” Annu. Rev. Phys. Chem.61(1), 345–367 (2010). [CrossRef] [PubMed]
- S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
- G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
- P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, “Online image analysis software for photoactivation localization microscopy,” Nat. Methods6(10), 689–690 (2009). [CrossRef] [PubMed]
- F. V. Subach, G. H. Patterson, S. Manley, J. M. Gillette, J. Lippincott-Schwartz, and V. V. Verkhusha, “Photoactivatable mCherry for high-resolution two-color fluorescence microscopy,” Nat. Methods6(2), 153–159 (2009). [CrossRef] [PubMed]
- B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science319(5864), 810–813 (2008). [CrossRef] [PubMed]
- M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods5(6), 527–529 (2008). [CrossRef] [PubMed]
- S. Ram, P. Prabhat, J. Chao, E. S. Ward, and R. J. Ober, “High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells,” Biophys. J.95(12), 6025–6043 (2008). [CrossRef] [PubMed]
- A. Sergé, N. Bertaux, H. Rigneault, and D. Marguet, “Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes,” Nat. Methods5(8), 687–694 (2008). [CrossRef] [PubMed]
- H. Bannai, S. Lévi, C. Schweizer, M. Dahan, and A. Triller, “Imaging the lateral diffusion of membrane molecules with quantum dots,” Nat. Protoc.1(6), 2628–2634 (2007). [CrossRef] [PubMed]
- Z. Kam, P. Kner, D. Agard, and J. W. Sedat, “Modelling the application of adaptive optics to wide-field microscope live imaging,” J. Microsc.226(1), 33–42 (2007). [CrossRef] [PubMed]
- S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006). [CrossRef] [PubMed]
- E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
- M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006). [CrossRef] [PubMed]
- M. Dahan, S. Lévi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller, “Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking,” Science302(5644), 442–445 (2003). [CrossRef] [PubMed]
- R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J.82(5), 2775–2783 (2002). [CrossRef] [PubMed]
- M. K. Cheezum, W. F. Walker, and W. H. Guilford, “Quantitative comparison of algorithms for tracking single fluorescent particles,” Biophys. J.81(4), 2378–2388 (2001). [CrossRef] [PubMed]
- M. A. Neil, R. Juskaitis, M. J. Booth, T. Wilson, T. Tanaka, and S. Kawata, “Adaptive aberration correction in a two-photon microscope,” J. Microsc.200(2), 105–108 (2000). [CrossRef] [PubMed]
- M. J. Booth, M. Neil, and T. Wilson, “Aberration correction for confocal imaging in refractive‐index‐mismatched media,” J. Microsc.192(2), 90–98 (1998). [CrossRef]
- H. P. Kao and A. S. Verkman, “Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position,” Biophys. J.67(3), 1291–1300 (1994). [CrossRef] [PubMed]
- S. Forrest, “Genetic algorithms: principles of natural selection applied to computation,” Science261(5123), 872–878 (1993). [CrossRef] [PubMed]
Cited By |
 Alert me when this paper is cited |
OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.
Multimedia
| Multimedia Files | Recommended Software |
| » Media 1: MOV (191 KB) | QuickTime |
| » Media 2: AVI (7854 KB) | QuickTime |
| » Media 3: AVI (4869 KB) | QuickTime |
| » Media 4: AVI (11424 KB) | QuickTime |
Related Journal Articles 
- Whole-field optically sectioned fluorescence lifetime imaging (OL)
- Evaluation of fitness parameters used in an iterative approach to aberration correction in optical sectioning microscopy (AO)
- Active focus locking in an optically sectioning microscope utilizing a deformable membrane mirror (OL)
- Applying SLODAR to measure aberrations in the eye (OE)
- In vivo measurement of erythrocyte velocity and retinal blood flow using adaptive optics scanning laser ophthalmoscopy (OE)
Related Conference Papers
- Extended-Resolution Reconstruction of Structured Illumination Microscopy Data
- All-Fiber Common-Path Fourier-Domain Optical Coherence Microscopy for 3-D in vivo Endoscopic Subcellular Imaging
- All-Fiber Common-Path Fourier-Domain Optical Coherence Microscopy for 3-D in vivo Endoscopic Subcellular Imaging
- A probe beam which encodes aberrations
- Adaptive Optics for High-Resolution Microscopy
« Previous Article | Next Article »
OSA is a member of 
For optimal display we recommend the following web browsers:
- Firefox 11+
- Google Chrome 17+
- Internet Explorer 9+
- Safari 5+