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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 5 — Feb. 27, 2012
  • pp: 5629–5635

Dynamic and high-resolution live cell imaging by direct electron beam excitation

Yasunori Nawa, Wataru Inami, Akito Chiba, Atsushi Ono, Atsuo Miyakawa, Yoshimasa Kawata, Sheng Lin, and Susumu Terakawa  »View Author Affiliations


Optics Express, Vol. 20, Issue 5, pp. 5629-5635 (2012)
http://dx.doi.org/10.1364/OE.20.005629


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Abstract

We propose a direct electron-beam excitation assisted optical microscope with a resolution of a few tens of nanometers and it can be applied for observation of dynamic movements of nanoparticles in liquid. The technique is also useful for live cell imaging under physiological conditions as well as observation of colloidal solution, microcrystal growth in solutions, etc. In the microscope, fluorescent materials are directly excited with a focused electron beam. The direct excitation with an electron beam yields high spatial resolution since the electron beam can be focused to a few tens of nanometers in the specimens. In order to demonstrate the potential of our proposed microscope, we observed the movements of fluorescent nanoparticles, which can be used for labelling specimens, in a water-based solution. We also demonstrated an observation result of living CHO cells.

© 2012 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0180) Medical optics and biotechnology : Microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 4, 2012
Revised Manuscript: February 2, 2012
Manuscript Accepted: February 8, 2012
Published: February 22, 2012

Virtual Issues
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Yasunori Nawa, Wataru Inami, Akito Chiba, Atsushi Ono, Atsuo Miyakawa, Yoshimasa Kawata, Sheng Lin, and Susumu Terakawa, "Dynamic and high-resolution live cell imaging by direct electron beam excitation," Opt. Express 20, 5629-5635 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-5-5629


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