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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 6 — Mar. 12, 2012
  • pp: 6693–6702

Optical microscope angular illumination analysis

Ravikiran Attota and Richard Silver  »View Author Affiliations

Optics Express, Vol. 20, Issue 6, pp. 6693-6702 (2012)

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For high precision applications of optical microscopes, it is critical to achieve symmetrical angular illumination intensity at the sample plane, in addition to uniform spatial irradiance achieved by Köhler illumination. A correlation between the angular illumination asymmetry and the contrast in the image of a line grating target was demonstrated as the target is scanned through focus. Using this correlation, we present a novel, yet simple method of experimentally evaluating the angular illumination asymmetry (ANILAS) at the sample plane of an optical microscope across the field of view. This ANILAS map is expected to be a useful method for assessing the illumination condition of optical systems.

© 2012 OSA

OCIS Codes
(180.0180) Microscopy : Microscopy
(220.1140) Optical design and fabrication : Alignment
(220.4840) Optical design and fabrication : Testing
(220.2945) Optical design and fabrication : Illumination design

ToC Category:

Original Manuscript: January 13, 2012
Revised Manuscript: February 27, 2012
Manuscript Accepted: February 28, 2012
Published: March 7, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Ravikiran Attota and Richard Silver, "Optical microscope angular illumination analysis," Opt. Express 20, 6693-6702 (2012)

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