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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 6 — Mar. 12, 2012
  • pp: 6737–6745

Gradient field microscopy of unstained specimens

Taewoo Kim, Shamira Sridharan, and Gabriel Popescu  »View Author Affiliations


Optics Express, Vol. 20, Issue 6, pp. 6737-6745 (2012)
http://dx.doi.org/10.1364/OE.20.006737


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Abstract

We present a phase derivative microscopy technique referred to as gradient field microscopy (GFM), which provides the first-order derivatives of the phase associated with an optical field passing through a transparent specimen. GFM utilizes spatial light modulation at the Fourier plane of a bright field microscope to optically obtain the derivatives of the phase and increase the contrast of the final image. The controllable spatial modulation pattern allows us to obtain both one component of the field gradient (derivative along one direction) and the gradient intensity, which offers some advantages over the regular differential interference contrast (DIC) microscopy. Most importantly, unlike DIC, GFM does not use polarizing optics and, thus, it is applicable to birefringent samples. We demonstrate these features of GFM with studies of static and dynamic biological cells (HeLa cells and red blood cells). We show that GFM is capable of qualitatively providing information about cell membrane fluctuations. Specifically, we captured the disappearance of the bending mode of fluctuations in osmotically swollen red blood cells.

© 2012 OSA

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1650) Medical optics and biotechnology : Coherence imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 2, 2012
Revised Manuscript: March 2, 2012
Manuscript Accepted: March 3, 2012
Published: March 7, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Taewoo Kim, Shamira Sridharan, and Gabriel Popescu, "Gradient field microscopy of unstained specimens," Opt. Express 20, 6737-6745 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-6-6737


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References

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