OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 7 — Mar. 26, 2012
  • pp: 7290–7299

Digistain: a digital staining instrument for histopathology

Hemmel Amrania, Giuseppe Antonacci, Che-Hung Chan, Laurence Drummond, William R Otto, Nicholas A. Wright, and Chris Phillips  »View Author Affiliations


Optics Express, Vol. 20, Issue 7, pp. 7290-7299 (2012)
http://dx.doi.org/10.1364/OE.20.007290


View Full Text Article

Enhanced HTML    Acrobat PDF (987 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We describe a new mid-infrared (mid-IR) imaging method specifically designed to augment the H + E tissue staining protocol. Images are taken with bespoke IR filters at wavelengths that enable chemical maps to be generated, corresponding to the cytoplasmic (amide) and nuclear (phosphodiester) components of unstained oesophageal tissue sections. A suitably calibrated combination of these generates false colour computer images that reproduce not only the tissue morphology, but also accurate and quantitative distributions of the nuclear-to-cytoplasmic ratio throughout the tissue section. This parameter is a well documented marker of malignancy, and because the images can be taken and interpreted by clinically trained personnel in a few seconds, we believe this new “digistain” approach makes spectroscopic mid-IR imaging techniques available for the first time as a practical, specific and sensitive augmentation to standard clinical cancer diagnosis methods.

© 2012 OSA

OCIS Codes
(110.3080) Imaging systems : Infrared imaging
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(100.4145) Image processing : Motion, hyperspectral image processing

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 2, 2011
Revised Manuscript: January 30, 2012
Manuscript Accepted: February 4, 2012
Published: March 15, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Hemmel Amrania, Giuseppe Antonacci, Che-Hung Chan, Laurence Drummond, William R Otto, Nicholas A. Wright, and Chris Phillips, "Digistain: a digital staining instrument for histopathology," Opt. Express 20, 7290-7299 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-7-7290


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. R. Salzer and H. W. Siesler Eds, (2009) ″Infrared and Raman Spectroscopic imaging″ Wiley-VCH, Weinheim ISBN 978–3-527- 31993–0.
  2. S. J. Jang, J. M. Gardner, and J. Y. Ro, “Diagnostic approach and prognostic factors of cancers,” Adv. Anat. Pathol.18(2), 165–172 (2011). [CrossRef] [PubMed]
  3. L. M. Merlo, L. S. Wang, J. W. Pepper, P. S. Rabinovitch, and C. C. Maley, “Polyploidy, aneuploidy and the evolution of cancer,” Adv. Exp. Med. Biol.676, 1–13 (2010). [CrossRef] [PubMed]
  4. L. Hoover and J. J. Berman, “Epithelial repair versus carcinoma in esophageal brush cytology,” Diagn. Cytopathol.4(3), 217–223 (1988). [CrossRef] [PubMed]
  5. C. N. Battlehner, P. H. Saldiva, C. R. Carvalho, T. Y. Takagaki, G. S. Montes, R. N. Younes, and V. L. Capelozzi, “Nuclear/cytoplasmic ratio correlates strongly with survival in non-disseminated neuroendocrine carcinoma of the lung,” Histopathology22(1), 31–34 (1993). [CrossRef] [PubMed]
  6. D. P. Coco, J. R. Goldblum, J. L. Hornick, G. Y. Lauwers, E. Montgomery, A. Srivastava, H. Wang, and R. D. Odze, “Interobserver variability in the diagnosis of crypt dysplasia in Barrett esophagus,” Am. J. Surg. Pathol.35(1), 45–54 (2011). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited