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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 7 — Mar. 26, 2012
  • pp: 7290–7299

Digistain: a digital staining instrument for histopathology

Hemmel Amrania, Giuseppe Antonacci, Che-Hung Chan, Laurence Drummond, William R Otto, Nicholas A. Wright, and Chris Phillips  »View Author Affiliations

Optics Express, Vol. 20, Issue 7, pp. 7290-7299 (2012)

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We describe a new mid-infrared (mid-IR) imaging method specifically designed to augment the H + E tissue staining protocol. Images are taken with bespoke IR filters at wavelengths that enable chemical maps to be generated, corresponding to the cytoplasmic (amide) and nuclear (phosphodiester) components of unstained oesophageal tissue sections. A suitably calibrated combination of these generates false colour computer images that reproduce not only the tissue morphology, but also accurate and quantitative distributions of the nuclear-to-cytoplasmic ratio throughout the tissue section. This parameter is a well documented marker of malignancy, and because the images can be taken and interpreted by clinically trained personnel in a few seconds, we believe this new “digistain” approach makes spectroscopic mid-IR imaging techniques available for the first time as a practical, specific and sensitive augmentation to standard clinical cancer diagnosis methods.

© 2012 OSA

OCIS Codes
(110.3080) Imaging systems : Infrared imaging
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(100.4145) Image processing : Motion, hyperspectral image processing

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 2, 2011
Revised Manuscript: January 30, 2012
Manuscript Accepted: February 4, 2012
Published: March 15, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Hemmel Amrania, Giuseppe Antonacci, Che-Hung Chan, Laurence Drummond, William R Otto, Nicholas A. Wright, and Chris Phillips, "Digistain: a digital staining instrument for histopathology," Opt. Express 20, 7290-7299 (2012)

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