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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 7 — Mar. 26, 2012
  • pp: 7323–7337

Incorporation of an experimentally determined MTF for spatial frequency filtering and deconvolution during optical projection tomography reconstruction

Lingling Chen, James McGinty, Harriet B. Taylor, Laurence Bugeon, Jonathan R. Lamb, Margaret J. Dallman, and Paul M. W. French  »View Author Affiliations

Optics Express, Vol. 20, Issue 7, pp. 7323-7337 (2012)

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We demonstrate two techniques to improve the quality of reconstructed optical projection tomography (OPT) images using the modulation transfer function (MTF) as a function of defocus experimentally determined from tilted knife-edge measurements. The first employs a 2-D binary filter based on the MTF frequency cut-off as an additional filter during back-projection reconstruction that restricts the high frequency information to the region around the focal plane and progressively decreases the spatial frequency bandwidth with defocus. This helps to suppress “streak” artifacts in OPT data acquired at reduced angular sampling, thereby facilitating faster OPT acquisitions. This method is shown to reduce the average background by approximately 72% for an NA of 0.09 and by approximately 38% for an NA of 0.07 compared to standard filtered back-projection. As a biological illustration, a Fli:GFP transgenic zebrafish embryo (3 days post-fertilisation) was imaged to demonstrate the improved imaging speed (a quarter of the acquisition time). The second method uses the MTF to produce an appropriate deconvolution filter that can be used to correct for the spatial frequency modulation applied by the imaging system.

© 2012 OSA

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 13, 2011
Revised Manuscript: January 13, 2012
Manuscript Accepted: January 30, 2012
Published: March 15, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Lingling Chen, James McGinty, Harriet B. Taylor, Laurence Bugeon, Jonathan R. Lamb, Margaret J. Dallman, and Paul M. W. French, "Incorporation of an experimentally determined MTF for spatial frequency filtering and deconvolution during optical projection tomography reconstruction," Opt. Express 20, 7323-7337 (2012)

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