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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 7 — Mar. 26, 2012
  • pp: 7338–7349

Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules

Saumya Saurabh, Suvrajit Maji, and Marcel P. Bruchez  »View Author Affiliations


Optics Express, Vol. 20, Issue 7, pp. 7338-7349 (2012)
http://dx.doi.org/10.1364/OE.20.007338


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Abstract

The ability to detect single molecules over the electronic noise requires high performance detector systems. Electron Multiplying Charge-Coupled Device (EMCCD) cameras have been employed successfully to image single molecules. Recently, scientific Complementary Metal Oxide Semiconductor (sCMOS) based cameras have been introduced with very low read noise at faster read out rates, smaller pixel sizes and a lower price compared to EMCCD cameras. In this study, we have compared the two technologies using two EMCCD and three sCMOS cameras to detect single Cy5 molecules. Our findings indicate that the sCMOS cameras perform similar to EMCCD cameras for detecting and localizing single Cy5 molecules.

© 2012 OSA

OCIS Codes
(110.2970) Imaging systems : Image detection systems
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Imaging Systems

History
Original Manuscript: January 4, 2012
Revised Manuscript: February 23, 2012
Manuscript Accepted: March 5, 2012
Published: March 15, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Saumya Saurabh, Suvrajit Maji, and Marcel P. Bruchez, "Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules," Opt. Express 20, 7338-7349 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-7-7338


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