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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 7 — Mar. 26, 2012
  • pp: 7646–7654

Comparative intrinsic optical signal imaging of wild-type and mutant mouse retinas

Qiu-Xiang Zhang, Youwen Zhang, Rong-Wen Lu, Yi-Chao Li, Steven J. Pittler, Timothy W. Kraft, and Xin-Cheng Yao  »View Author Affiliations


Optics Express, Vol. 20, Issue 7, pp. 7646-7654 (2012)
http://dx.doi.org/10.1364/OE.20.007646


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Abstract

Functional measurement is important for retinal study and disease diagnosis. Transient intrinsic optical signal (IOS) response, tightly correlated with functional stimulation, has been previously detected in normal retinas. In this paper, comparative IOS imaging of wild-type (WT) and rod-degenerated mutant mouse retinas is reported. Both 2-month and 1-year-old mice were measured. In 2-month-old mutant mice, time course and peak value of the stimulus-evoked IOS were significantly delayed (relative to stimulus onset) and reduced, respectively, compared to age matched WT mice. In 1-year-old mutant mice, stimulus-evoked IOS was totally absent. However, enhanced spontaneous IOS responses, which might reflect inner neural remodeling in diseased retina, were observed in both 2-month and 1-year-old mutant retinas. Our experiments demonstrate the potential of using IOS imaging for noninvasive and high resolution identification of disease-associated retinal dysfunctions. Moreover, high spatiotemporal resolution IOS imaging may also lead to advanced understanding of disease-associated neural remodeling in the retina.

© 2012 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(330.5380) Vision, color, and visual optics : Physiology
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 12, 2011
Revised Manuscript: January 19, 2012
Manuscript Accepted: February 4, 2012
Published: March 20, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Qiu-Xiang Zhang, Youwen Zhang, Rong-Wen Lu, Yi-Chao Li, Steven J. Pittler, Timothy W. Kraft, and Xin-Cheng Yao, "Comparative intrinsic optical signal imaging of wild-type and mutant mouse retinas," Opt. Express 20, 7646-7654 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-7-7646


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