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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 8 — Apr. 9, 2012
  • pp: 8824–8836

High dynamic range optical projection tomography (HDR-OPT)

Peng Fei, Zhilong Yu, Xu Wang, Peter J. Lu, Yusi Fu, Zi He, Jingwei Xiong, and Yanyi Huang  »View Author Affiliations

Optics Express, Vol. 20, Issue 8, pp. 8824-8836 (2012)

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Traditional optical projection tomography (OPT) acquires a single image at each rotation angle, thereby suffering from limitations in CCD dynamic range; this conventional usage cannot resolve features in samples with highly heterogeneous absorption, such as in small animals with organs of varying size. We present a novel technique, applying multiple-exposure high dynamic range (HDR) imaging to OPT, and demonstrate its ability to resolve fine details in zebrafish embryos, without complicated chemical clearing. We implement the tomographic reconstruction algorithm on the GPU, yielding a performance increase of two orders of magnitude. These features give our method potential application in high-throughput, high-resolution in vivo 3D imaging.

© 2012 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 5, 2012
Revised Manuscript: March 8, 2012
Manuscript Accepted: March 29, 2012
Published: April 2, 2012

Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

Peng Fei, Zhilong Yu, Xu Wang, Peter J. Lu, Yusi Fu, Zi He, Jingwei Xiong, and Yanyi Huang, "High dynamic range optical projection tomography (HDR-OPT)," Opt. Express 20, 8824-8836 (2012)

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