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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 8 — Apr. 9, 2012
  • pp: 9203–9214

Real-time quantitative visualization of 3D structural information

Sergey A. Alexandrov, Shikhar Uttam, Rajan K. Bista, Chengquan Zhao, and Yang Liu  »View Author Affiliations


Optics Express, Vol. 20, Issue 8, pp. 9203-9214 (2012)
http://dx.doi.org/10.1364/OE.20.009203


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Abstract

We demonstrate a novel approach for the real time visualization and quantification of the 3D spatial frequencies in an image domain. Our approach is based on the spectral encoding of spatial frequency principle and permits the formation of an image as a color map in which spatially separated spectral wavelengths correspond to the dominant 3D spatial frequencies of the object. We demonstrate that our approach can visualize and analyze the dominant axial internal structure for each image point in real time and with nanoscale sensitivity to structural changes. Computer modeling and experimental results of instantaneous color visualization and quantification of 3D structures of a model system and biological samples are presented.

© 2012 OSA

OCIS Codes
(070.4790) Fourier optics and signal processing : Spectrum analysis
(070.6110) Fourier optics and signal processing : Spatial filtering
(180.6900) Microscopy : Three-dimensional microscopy
(290.1350) Scattering : Backscattering
(050.6624) Diffraction and gratings : Subwavelength structures

ToC Category:
Fourier Optics and Signal Processing

History
Original Manuscript: February 29, 2012
Revised Manuscript: March 26, 2012
Manuscript Accepted: March 30, 2012
Published: April 5, 2012

Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Sergey A. Alexandrov, Shikhar Uttam, Rajan K. Bista, Chengquan Zhao, and Yang Liu, "Real-time quantitative visualization of 3D structural information," Opt. Express 20, 9203-9214 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-8-9203


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