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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 8 — Apr. 9, 2012
  • pp: 9276–9283

Short time behavior of fluorescence intensity fluctuations in single molecule polarization sensitive experiments

Lior Turgeman and Dror Fixler  »View Author Affiliations

Optics Express, Vol. 20, Issue 8, pp. 9276-9283 (2012)

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Recent developments in the field of single molecule orientation imaging have led us to devise a simple framework for analyzing fluorescence intensity fluctuations in single molecule polarization sensitive experiments. Based on the new framework, rotational dynamics of individual molecules are quantified, in this paper, from the short time behavior of the time averaged fluorescence intensity fluctuation trajectories. The suggested model can be applied in single molecule fluorescence fluctuations experiments to extract accurate expectation values of photon counts during very short integration time in which rotational diffusion is likely not to be averaged out.

© 2012 OSA

OCIS Codes
(000.5490) General : Probability theory, stochastic processes, and statistics
(030.5260) Coherence and statistical optics : Photon counting
(110.4280) Imaging systems : Noise in imaging systems
(180.2520) Microscopy : Fluorescence microscopy
(260.5430) Physical optics : Polarization
(270.5290) Quantum optics : Photon statistics

ToC Category:

Original Manuscript: February 2, 2012
Revised Manuscript: April 2, 2012
Manuscript Accepted: April 2, 2012
Published: April 6, 2012

Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

Lior Turgeman and Dror Fixler, "Short time behavior of fluorescence intensity fluctuations in single molecule polarization sensitive experiments," Opt. Express 20, 9276-9283 (2012)

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