OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 9 — Apr. 23, 2012
  • pp: 9673–9681

Optics InfoBase > Optics Express > Volume 20 > Issue 9 > Page 9673

Dynamic spectroscopic phase microscopy for quantifying hemoglobin concentration and dynamic membrane fluctuation in red blood cells

Yunhun Jang, Jaeduck Jang, and YongKeun Park  »View Author Affiliations


Optics Express, Vol. 20, Issue 9, pp. 9673-9681 (2012)
http://dx.doi.org/10.1364/OE.20.009673


View Full Text Article

Enhanced HTML    Acrobat PDF (966 KB)


Advanced Search

Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We report a technique for simultaneous label-free quantification of cytoplasmic hemoglobin Hb concentration and dynamic membrane fluctuation in individual red blood cells (RBCs). Spectroscopic phase microscopy equipped with three different coherent laser sources and a color detector records three wavelength-dependent quantitative phase images in a single shot of a color-coded hologram. Using molecular specific dispersion, we demonstrate the extraction of Hb concentration and the dynamic membrane fluctuation from individual RBCs.

© 2012 OSA

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 22, 2012
Revised Manuscript: April 5, 2012
Manuscript Accepted: April 5, 2012
Published: April 12, 2012

Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Yunhun Jang, Jaeduck Jang, and YongKeun Park, "Dynamic spectroscopic phase microscopy for quantifying hemoglobin concentration and dynamic membrane fluctuation in red blood cells," Opt. Express 20, 9673-9681 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-9-9673


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. V. Ntziachristos, “Fluorescence molecular imaging,” Annu. Rev. Biomed. Eng.8(1), 1–33 (2006). [CrossRef] [PubMed]
  2. S. Keren, C. Zavaleta, Z. Cheng, A. de la Zerda, O. Gheysens, and S. S. Gambhir, “Noninvasive molecular imaging of small living subjects using Raman spectroscopy,” Proc. Natl. Acad. Sci. U.S.A.105(15), 5844–5849 (2008). [CrossRef] [PubMed]
  3. J. Stevens and W. Baier, “Refractometric determination of soluble solids in citrus juices,” Ind. Eng. Chem. Anal. Ed.11(8), 447–449 (1939). [CrossRef]
  4. J. Homola, S. S. Yee, and G. Gauglitz, “Surface plasmon resonance sensors: review,” Sens. Actuators B Chem.54(1-2), 3–15 (1999). [CrossRef]
  5. G. Popescu, Quantitative Phase Imaging of Cells and Tissues (McGraw-Hill Professional, 2011).
  6. B. Kemper and G. von Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt.47(4), A52–A61 (2008). [CrossRef] [PubMed]
  7. B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer,” Cytometry A73A(10), 895–903 (2008). [CrossRef] [PubMed]
  8. B. Rappaz, F. Charrière, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium,” Opt. Lett.33(7), 744–746 (2008). [CrossRef] [PubMed]
  9. M. K. Kim, Digital Holography and Microscopy: Principles, Techniques, and Applications (Springer Verlag, 2011).
  10. Y.-K. Park, W. Choi, Z. Yaqoob, R. Dasari, K. Badizadegan, and M. S. Feld, “Speckle-field digital holographic microscopy,” Opt. Express17(15), 12285–12292 (2009). [CrossRef] [PubMed]
  11. M. S. Amin, Y. Park, N. Lue, R. R. Dasari, K. Badizadegan, M. S. Feld, and G. Popescu, “Microrheology of red blood cell membranes using dynamic scattering microscopy,” Opt. Express15(25), 17001–17009 (2007). [CrossRef] [PubMed]
  12. S. K. Debnath and Y. Park, “Real-time quantitative phase imaging with a spatial phase-shifting algorithm,” Opt. Lett.36(23), 4677–4679 (2011). [CrossRef] [PubMed]
  13. Z. Yaqoob, W. Choi, S. Oh, N. Lue, Y. Park, C. Fang-Yen, R. R. Dasari, K. Badizadegan, and M. S. Feld, “Improved phase sensitivity in spectral domain phase microscopy using line-field illumination and self phase-referencing,” Opt. Express17(13), 10681–10687 (2009). [CrossRef] [PubMed]
  14. S. Bernet, A. Jesacher, S. Fürhapter, C. Maurer, and M. Ritsch-Marte, “Quantitative imaging of complex samples by spiral phase contrast microscopy,” Opt. Express14(9), 3792–3805 (2006). [CrossRef] [PubMed]
  15. I. Yamaguchi, T. Matsumura, and J. Kato, “Phase-shifting color digital holography,” Opt. Lett.27(13), 1108–1110 (2002). [CrossRef] [PubMed]
  16. G. Popescu, Y. Park, N. Lue, C. Best-Popescu, L. Deflores, R. R. Dasari, M. S. Feld, and K. Badizadegan, “Optical imaging of cell mass and growth dynamics,” Am. J. Physiol. Cell Physiol.295(2), C538–C544 (2008). [CrossRef] [PubMed]
  17. M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011). [CrossRef] [PubMed]
  18. H. F. Ding, Z. Wang, F. Nguyen, S. A. Boppart, and G. Popescu, “Fourier transform light scattering of inhomogeneous and dynamic structures,” Phys. Rev. Lett.101(23), 238102 (2008). [CrossRef] [PubMed]
  19. Y. Park, C. A. Best-Popescu, R. R. Dasari, and G. Popescu, “Light scattering of human red blood cells during metabolic remodeling of the membrane,” J. Biomed. Opt.16(1), 011013 (2011). [CrossRef] [PubMed]
  20. Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, and M. S. Feld, “Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells,” J. Biomed. Opt.15(2), 020506 (2010). [CrossRef] [PubMed]
  21. D. Fu, W. Choi, Y. Sung, S. Oh, Z. Yaqoob, Y. Park, R. R. Dasari, and M. S. Feld, “Ultraviolet refractometry using field-based light scattering spectroscopy,” Opt. Express17(21), 18878–18886 (2009). [CrossRef] [PubMed]
  22. Y. Park, C. A. Best, K. Badizadegan, R. R. Dasari, M. S. Feld, T. Kuriabova, M. L. Henle, A. J. Levine, and G. Popescu, “Measurement of red blood cell mechanics during morphological changes,” Proc. Natl. Acad. Sci. U.S.A.107(15), 6731–6736 (2010). [CrossRef] [PubMed]
  23. Y. K. Park, C. A. Best, T. Auth, N. S. Gov, S. A. Safran, G. Popescu, S. Suresh, and M. S. Feld, “Metabolic remodeling of the human red blood cell membrane,” Proc. Natl. Acad. Sci. U.S.A.107(4), 1289–1294 (2010). [CrossRef] [PubMed]
  24. Y. K. Park, C. A. Best, T. Kuriabova, M. L. Henle, M. S. Feld, A. J. Levine, and G. Popescu, “Measurement of the nonlinear elasticity of red blood cell membranes,” Phys. Rev. E Stat. Nonlin. Soft Matter Phys.83(5), 051925 (2011). [CrossRef] [PubMed]
  25. Y.-K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A.105(37), 13730–13735 (2008). [CrossRef] [PubMed]
  26. G. Popescu, Y. K. Park, R. R. Dasari, K. Badizadegan, and M. S. Feld, “Coherence properties of red blood cell membrane motions,” Phys. Rev. E Stat. Nonlin. Soft Matter Phys.76(3), 031902 (2007). [CrossRef] [PubMed]
  27. R. Chandramohanadas, Y. K. Park, L. Lui, A. Li, D. Quinn, K. Liew, M. Diez-Silva, Y. Sung, M. Dao, C. T. Lim, P. R. Preiser, and S. Suresh, “Biophysics of malarial parasite exit from infected erythrocytes,” PLoS ONE6(6), e20869 (2011). [CrossRef] [PubMed]
  28. Z. Wang, L. Millet, M. Mir, H. Ding, S. Unarunotai, J. Rogers, M. U. Gillette, and G. Popescu, “Spatial light interference microscopy (SLIM),” Opt. Express19(2), 1016–1026 (2011). [CrossRef] [PubMed]
  29. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4(9), 717–719 (2007). [CrossRef] [PubMed]
  30. J. Garay, J. A. D’Angelo, Y. K. Park, C. M. Summa, M. L. Aiken, E. Morales, K. Badizadegan, E. Fiebiger, and B. L. Dickinson, “Crosstalk between PKA and Epac regulates the phenotypic maturation and function of human dendritic cells,” J. Immunol.185(6), 3227–3238 (2010). [CrossRef] [PubMed]
  31. P. Jourdain, N. Pavillon, C. Moratal, D. Boss, B. Rappaz, C. Depeursinge, P. Marquet, and P. J. Magistretti, “Determination of transmembrane water fluxes in neurons elicited by glutamate ionotropic receptors and by the cotransporters KCC2 and NKCC1: a digital holographic microscopy study,” J. Neurosci.31(33), 11846–11854 (2011). [CrossRef] [PubMed]
  32. B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt.14(3), 034049 (2009). [CrossRef] [PubMed]
  33. A. Yusipovich, E. Y. Parshina, N. Y. Brysgalova, A. Brazhe, N. Brazhe, A. Lomakin, G. Levin, and G. Maksimov, “Laser interference microscopy in erythrocyte study,” J. Appl. Phys.105(10), 102037 (2009). [CrossRef]
  34. C. Yang, A. Wax, I. Georgakoudi, E. B. Hanlon, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Interferometric phase-dispersion microscopy,” Opt. Lett.25(20), 1526–1528 (2000). [CrossRef] [PubMed]
  35. Y. Park, T. Yamauchi, W. Choi, R. Dasari, and M. S. Feld, “Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells,” Opt. Lett.34(23), 3668–3670 (2009). [CrossRef] [PubMed]
  36. D. Fu, W. Choi, Y. Sung, Z. Yaqoob, R. R. Dasari, and M. Feld, “Quantitative dispersion microscopy,” Biomed. Opt. Express1(2), 347–353 (2010). [CrossRef] [PubMed]
  37. M. Mir, K. Tangella, and G. Popescu, “Blood testing at the single cell level using quantitative phase and amplitude microscopy,” Biomed. Opt. Express2(12), 3259–3266 (2011). [CrossRef] [PubMed]
  38. F. E. Robles, L. L. Satterwhite, and A. Wax, “Non-linear phase dispersion spectroscopy,” Opt. Lett.36, 4665–4667 (2011).
  39. O. Zhernovaya, O. Sydoruk, V. Tuchin, and A. Douplik, “The refractive index of human hemoglobin in the visible range,” Phys. Med. Biol.56(13), 4013–4021 (2011). [CrossRef] [PubMed]
  40. M. Friebel and M. Meinke, “Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration,” Appl. Opt.45(12), 2838–2842 (2006). [CrossRef] [PubMed]
  41. Y. K. Park, G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Diffraction phase and fluorescence microscopy,” Opt. Express14(18), 8263–8268 (2006). [CrossRef] [PubMed]
  42. G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, “Diffraction phase microscopy for quantifying cell structure and dynamics,” Opt. Lett.31(6), 775–777 (2006). [CrossRef] [PubMed]
  43. J. M. Desse, P. Picart, and P. Tankam, “Digital three-color holographic interferometry for flow analysis,” Opt. Express16(8), 5471–5480 (2008). [CrossRef] [PubMed]
  44. N. Demoli, D. Vukicevic, and M. Torzynski, “Dynamic digital holographic interferometry with three wavelengths,” Opt. Express11(7), 767–774 (2003). [CrossRef] [PubMed]
  45. P. S. Huang, Q. Hu, F. Jin, and F. P. Chiang, “Color-encoded digital fringe projection technique for high-speed three-dimensional surface contouring,” Opt. Eng.38(6), 1065 (1999). [CrossRef]
  46. L. G. Krzewina and M. K. Kim, “Single-exposure optical sectioning by color structured illumination microscopy,” Opt. Lett.31(4), 477–479 (2006). [CrossRef] [PubMed]
  47. Y. K. Park, C. A. Best, and G. Popescu, “Optical Sensing of Red Blood Cell Dynamics,” in Mechanobiology of Cell-cell and Cell-matrix Interactions(Springer, 2011), p. 279.
  48. G. Popescu, Y. Park, W. Choi, R. R. Dasari, M. S. Feld, and K. Badizadegan, “Imaging red blood cell dynamics by quantitative phase microscopy,” Blood Cells Mol. Dis.41(1), 10–16 (2008). [CrossRef] [PubMed]
  49. G. A. Barabino, M. O. Platt, and D. K. Kaul, “Sickle cell biomechanics,” Annu. Rev. Biomed. Eng.12(1), 345–367 (2010). [CrossRef] [PubMed]
  50. Y. Kim, J. Higgins, R. Dasari, S. Suresh, and Y.-K. Park, “Light scatting spectroscopy of individla red blood cells from a sickle cell patient using Fourier Transfrom Light Scattering method,” J. Biomed. Opt. Lett. in press.
  51. S. Cho, S. Kim, Y. Kim, and Y. K. Park, “Optical imaging techniques for the study of malaria,” Trends Biotechnol.30(2), 71–79 (2012). [CrossRef] [PubMed]

Cited By

 Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4 Fig. 5
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited