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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 10 — May. 20, 2013
  • pp: 12579–12591

Optimization of excitation–emission band-pass filter for visualization of viable bacteria distribution on the surface of pork meat

Ken Nishino, Kazuaki Nakamura, Mizuki Tsuta, Masatoshi Yoshimura, Junichi Sugiyama, and Shigeki Nakauchi  »View Author Affiliations

Optics Express, Vol. 21, Issue 10, pp. 12579-12591 (2013)

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A novel method of optically reducing the dimensionality of an excitation–emission matrix (EEM) by optimizing the excitation and emission band-pass filters was proposed and applied to the visualization of viable bacteria on pork. Filters were designed theoretically using an EEM data set for evaluating colony-forming units on pork samples assuming signal-to-noise ratios of 100, 316, or 1000. These filters were evaluated using newly measured EEM images. The filters designed for S/N = 100 performed the best and allowed the visualization of viable bacteria distributions. The proposed method is expected to be a breakthrough in the application of EEM imaging.

© 2013 OSA

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(120.2440) Instrumentation, measurement, and metrology : Filters
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Imaging Systems

Original Manuscript: March 12, 2013
Revised Manuscript: April 29, 2013
Manuscript Accepted: April 29, 2013
Published: May 15, 2013

Virtual Issues
Vol. 8, Iss. 6 Virtual Journal for Biomedical Optics

Ken Nishino, Kazuaki Nakamura, Mizuki Tsuta, Masatoshi Yoshimura, Junichi Sugiyama, and Shigeki Nakauchi, "Optimization of excitation–emission band-pass filter for visualization of viable bacteria distribution on the surface of pork meat," Opt. Express 21, 12579-12591 (2013)

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