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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 12 — Jun. 17, 2013
  • pp: 14474–14480

A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis

Jianglai Wu, Jianping Li, and Robert K.Y. Chan  »View Author Affiliations


Optics Express, Vol. 21, Issue 12, pp. 14474-14480 (2013)
http://dx.doi.org/10.1364/OE.21.014474


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Abstract

This paper reports a light sheet fluorescence imaging flow cytometer for 3D sectioning of phytoplankton. The instrument developed has the inherent advantages of high cell counting throughput and high spatial resolution information derived from flow cytometry and light sheet microscopy. The throughput of the instrument is quantified by the sample volume flow rate of 0.5 μl/min with a spatial resolution as achieved by light sheet microscopy. Preliminary results from 3D morphology of the internal chlorophyll-a structure of two dinoflagellates species show promising application potentials of the method for phytoplankton taxonomy of selected species and species groups.

© 2013 OSA

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(110.0110) Imaging systems : Imaging systems
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Atmospheric and Oceanic Optics

History
Original Manuscript: April 29, 2013
Revised Manuscript: May 29, 2013
Manuscript Accepted: May 31, 2013
Published: June 10, 2013

Virtual Issues
Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Jianglai Wu, Jianping Li, and Robert K.Y. Chan, "A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis," Opt. Express 21, 14474-14480 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-12-14474


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References

  1. Z. V. Finkel, J. Beardall, K. J. Flynn, A. Quigg, T. A. V. Rees, and J. A. Raven, “Phytoplankton in a changing world: cell size and elemental stoichiometry,” J. Plankton Res.32(1), 119–137 (2010). [CrossRef]
  2. L. Bonetta, “Flow cytometry smaller and better,” Nat. Methods2(10), 785–795 (2005). [CrossRef]
  3. D. A. Basiji, W. E. Ortyn, L. Liang, V. Venkatachalam, and P. Morrissey, “Cellular image analysis and imaging by flow cytometry,” Clin. Lab. Med.27(3), 653–670, viii (2007). [CrossRef] [PubMed]
  4. . F. Culverhouse, R. Williams, M. Benfield, P. R. Flood, A. F. Sell, M. G. Mazzocchi, I. Buttino, and M. Sieracki, “Automatic image analysis of plankton: future perspectives,” Mar. Ecol. Prog. Ser.312, 297–309 (2006). [CrossRef]
  5. R. Boistel, J. Swoger, U. Kržič, V. Fernandez, B. Gillet, and E. G. Reynaud, “The future of three-dimensional microscopic imaging in marine biology,” Mar. Ecol. (Berl.)32(4), 438–452 (2011). [CrossRef]
  6. J. A. Conchello and J. W. Lichtman, “Optical sectioning microscopy,” Nat. Methods2(12), 920–931 (2005). [CrossRef] [PubMed]
  7. E. G. Reynaud, U. Kržič, K. Greger, and E. H. K. Stelzer, “Light sheet-based fluorescence microscopy: more dimensions, more photons, and less photodamage,” HFSP J2(5), 266–275 (2008). [CrossRef] [PubMed]
  8. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
  9. L. Silvestri, A. Bria, L. Sacconi, G. Iannello, and F. S. Pavone, “Confocal light sheet microscopy: micron-scale neuroanatomy of the entire mouse brain,” Opt. Express20(18), 20582–20598 (2012). [CrossRef] [PubMed]
  10. M. Weber and J. Huisken, “Light sheet microscopy for real-time developmental biology,” Curr. Opin. Genet. Dev.21(5), 566–572 (2011). [CrossRef] [PubMed]
  11. J. Huisken and D. Y. R. Stainier, “Selective plane illumination microscopy techniques in developmental biology,” Development136(12), 1963–1975 (2009). [CrossRef] [PubMed]
  12. K. Khairy and P. J. Keller, “Reconstructing embryonic development,” Genesis49(7), 488–513 (2011). [CrossRef] [PubMed]
  13. S. Kumar, D. Wilding, M. B. Sikkel, A. R. Lyon, K. T. MacLeod, and C. Dunsby, “High-speed 2D and 3D fluorescence microscopy of cardiac myocytes,” Opt. Express19(15), 13839–13847 (2011). [CrossRef] [PubMed]
  14. K. Greger, J. Swoger, and E. H. K. Stelzer, “Basic building units and properties of a fluorescence single plane illumination microscope,” Rev. Sci. Instrum.78(2), 023705 (2007). [CrossRef] [PubMed]
  15. H. M. Shapiro, Practical flow cytometry (Wiley-Liss, 1993), Chapter. 4.
  16. C. J. Engelbrecht and E. H. K. Stelzer, “Resolution enhancement in a light-sheet-based microscope (SPIM),” Opt. Lett.31(10), 1477–1479 (2006). [CrossRef] [PubMed]
  17. E. Fuchs, J. S. Jaffe, R. A. Long, and F. Azam, “Thin laser light sheet microscope for microbial oceanography,” Opt. Express10(2), 145–154 (2002). [CrossRef] [PubMed]
  18. J. Huisken and D. Y. R. Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett.32(17), 2608–2610 (2007). [CrossRef] [PubMed]

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