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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 12 — Jun. 17, 2013
  • pp: 14474–14480

A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis

Jianglai Wu, Jianping Li, and Robert K.Y. Chan  »View Author Affiliations

Optics Express, Vol. 21, Issue 12, pp. 14474-14480 (2013)

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This paper reports a light sheet fluorescence imaging flow cytometer for 3D sectioning of phytoplankton. The instrument developed has the inherent advantages of high cell counting throughput and high spatial resolution information derived from flow cytometry and light sheet microscopy. The throughput of the instrument is quantified by the sample volume flow rate of 0.5 μl/min with a spatial resolution as achieved by light sheet microscopy. Preliminary results from 3D morphology of the internal chlorophyll-a structure of two dinoflagellates species show promising application potentials of the method for phytoplankton taxonomy of selected species and species groups.

© 2013 OSA

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(110.0110) Imaging systems : Imaging systems
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Atmospheric and Oceanic Optics

Original Manuscript: April 29, 2013
Revised Manuscript: May 29, 2013
Manuscript Accepted: May 31, 2013
Published: June 10, 2013

Virtual Issues
Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics

Jianglai Wu, Jianping Li, and Robert K.Y. Chan, "A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis," Opt. Express 21, 14474-14480 (2013)

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