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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 12 — Jun. 17, 2013
  • pp: 14555–14565

Wide field-of-view Talbot grid-based microscopy for multicolor fluorescence imaging

Shuo Pang, Chao Han, Jessey Erath, Ana Rodriguez, and Changhuei Yang  »View Author Affiliations

Optics Express, Vol. 21, Issue 12, pp. 14555-14565 (2013)

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The capability to perform multicolor, wide field-of-view (FOV) fluorescence microscopy imaging is important in screening and pathology applications. We developed a microscopic slide-imaging system that can achieve multicolor, wide FOV, fluorescence imaging based on the Talbot effect. In this system, a light-spot grid generated by the Talbot effect illuminates the sample. By tilting the excitation beam, the Talbot-focused spot scans across the sample. The images are reconstructed by collecting the fluorescence emissions that correspond to each focused spot with a relay optics arrangement. The prototype system achieved an FOV of 12 × 10 mm2 at an acquisition time as fast as 23 s for one fluorescence channel. The resolution is fundamentally limited by spot size, with a demonstrated full-width at half-maximum spot diameter of 1.2 μm. The prototype was used to nimage green fluorescent beads, double-stained human breast cancer SK-BR-3 cells, Giardia lamblia cysts, and the Cryptosporidium parvum oocysts. This imaging method is scalable and simple for implementation of high-speed wide FOV fluorescence microscopy.

© 2013 OSA

OCIS Codes
(110.6760) Imaging systems : Talbot and self-imaging effects
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:

Original Manuscript: May 14, 2013
Revised Manuscript: June 5, 2013
Manuscript Accepted: June 5, 2013
Published: June 11, 2013

Virtual Issues
Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics

Shuo Pang, Chao Han, Jessey Erath, Ana Rodriguez, and Changhuei Yang, "Wide field-of-view Talbot grid-based microscopy for multicolor fluorescence imaging," Opt. Express 21, 14555-14565 (2013)

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