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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 12 — Jun. 17, 2013
  • pp: 14636–14642

High frequency photoacoustic imaging for in vivo visualizing blood flow of zebrafish heart

Jinhyoung Park, Thomas M. Cummins, Michael Harrison, Jungwoo Lee, Qifa Zhou, Ching-Ling Lien, and K. Kirk Shung  »View Author Affiliations

Optics Express, Vol. 21, Issue 12, pp. 14636-14642 (2013)

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A technique on high frame rate(28fps), high frequency co-registered ultrasound and photoacoustic imaging for visualizing zebrafish heart blood flow was demonstrated. This approach was achieved with a 40MHz light weight(0.38g) ring-type transducer, serving as the ultrasound transmitter and receiver, to allow an optic fiber, coupled with a 532nm laser, to be inserted into the hole. From the wire target study, axial resolutions of 38µm and 42µm were obtained for ultrasound and photoacoustic imaging, respectively. Carbon nanotubes were utilized as contrast agents to increase the flow signal level by 20dB in phantom studies, and zebrafish heart blood flow was successfully observed.

© 2013 OSA

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(110.5120) Imaging systems : Photoacoustic imaging

ToC Category:
Imaging Systems

Original Manuscript: April 4, 2013
Revised Manuscript: May 23, 2013
Manuscript Accepted: May 31, 2013
Published: June 12, 2013

Virtual Issues
Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics

Jinhyoung Park, Thomas M. Cummins, Michael Harrison, Jungwoo Lee, Qifa Zhou, Ching-Ling Lien, and K. Kirk Shung, "High frequency photoacoustic imaging for in vivo visualizing blood flow of zebrafish heart," Opt. Express 21, 14636-14642 (2013)

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  1. K. D. Poss, L. G. Wilson, and M. T. Keating, “Heart regeneration in Zebrafish,” Science298(5601), 2188–2190 (2002). [CrossRef] [PubMed]
  2. J. R. Hove, “In vivo biofluid dynamic imaging in the developing Zebrafish,” Birth Defects Res. C Embryo Today72(3Part C), 277–289 (2004). [CrossRef] [PubMed]
  3. J. Park, Y. Huang, R. Chen, J. Lee, T. M. Cummins, Q. Zhou, C. L. Lien, and K. K. Shung, “Pulse Inversion Chirp Coded Tissue Harmonic Imaging (PI-CTHI) of zebrafish heart using high frame rate ultrasound biomicroscopy,” Ann. Biomed. Eng.41(1), 41–52 (2013). [CrossRef] [PubMed]
  4. L. Sun, X. Xu, W. D. Richard, C. Feng, J. A. Johnson, and K. K. Shung, “A high-frame rate duplex ultrasound biomicroscopy for small animal imaging in vivo,” IEEE Trans. Biomed. Eng.55(8), 2039–2049 (2008). [CrossRef] [PubMed]
  5. J. M. Yang, C. Favazza, R. Chen, J. Yao, X. Cai, K. Maslov, Q. Zhou, K. K. Shung, and L. V. Wang, “Simultaneous functional photoacoustic and ultrasonic endoscopy of internal organs in vivo,” Nat. Med.18(8), 1297–1302 (2012). [CrossRef] [PubMed]
  6. T. Harrison, J. C. Ranasinghesagara, H. Lu, K. Mathewson, A. Walsh, and R. J. Zemp, “Combined photoacoustic and ultrasound biomicroscopy,” Opt. Express17(24), 22041–22046 (2009). [CrossRef] [PubMed]
  7. Y. Zhang, H. Hong, and W. Cai, “Photoacoustic imaging,” Cold Spring Harb Protoc2011(9), 1015–1025 (2011). [CrossRef] [PubMed]
  8. J. Park, C. Hu, and K. K. Shung, “Stand-alone front-end system for high- frequency, high-frame-rate coded excitation ultrasonic imaging,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control58(12), 2620–2630 (2011). [CrossRef] [PubMed]
  9. J. M. Cannata, T. A. Ritter, W. H. Chen, R. H. Silverman, and K. K. Shung, “Design of efficient, broadband single-element (20-80 MHz) ultrasonic transducers for medical imaging applications,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control50(11), 1548–1557 (2003). [CrossRef] [PubMed]
  10. E. K. Pugach, P. Li, R. White, and L. Zon, “Retro-orbital injection in adult zebrafish,” J. Vis. Exp.34(34), 1645 (2009). [PubMed]

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