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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 14 — Jul. 15, 2013
  • pp: 16282–16289

Mapping of phase singularities with spiral phase contrast microscopy

Ruth Steiger, Stefan Bernet, and Monika Ritsch-Marte  »View Author Affiliations

Optics Express, Vol. 21, Issue 14, pp. 16282-16289 (2013)

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In spiral phase contrast (SPC) microscopy the edge-enhancement is typically independent of the helicity of the phase vortex filter. Here we show that for layered specimens containing screw-dislocations, as are e.g. present in mica or some crystallized organic substances, the intensity distribution in the filtered image acquires a dependence on the rotational direction of the filter. This allows one to map the distribution of phase singularities in the topography of the sample, by taking the intensity difference between two images recorded with opposite handedness. For the demonstration of this feature in a microscopy set-up, we encode the vortex filter as a binary off-axis hologram displayed on a spatial light modulator (SLM) placed in a Fourier plane. Using a binary grating, the diffraction efficiencies for the plus and minus first diffraction orders are equal, giving rise to two image waves which travel in different directions and are Fourier filtered with opposite helicity. The corresponding two images can be recorded simultaneously in two separate regions of the camera chip. This enables mapping of dislocations in the sample in a single camera exposure, as was demonstrated for various transparent samples.

© 2013 OSA

OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(170.0180) Medical optics and biotechnology : Microscopy
(230.3720) Optical devices : Liquid-crystal devices

ToC Category:

Original Manuscript: May 9, 2013
Revised Manuscript: June 19, 2013
Manuscript Accepted: June 24, 2013
Published: July 1, 2013

Virtual Issues
Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

Ruth Steiger, Stefan Bernet, and Monika Ritsch-Marte, "Mapping of phase singularities with spiral phase contrast microscopy," Opt. Express 21, 16282-16289 (2013)

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