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Multi-beam confocal microscopy based on a custom image sensor with focal-plane pinhole array effect |
Optics Express, Vol. 21, Issue 2, pp. 1417-1429 (2013)
http://dx.doi.org/10.1364/OE.21.001417
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Abstract
Multi-beam confocal microscopy without any physical pinhole was demonstrated. As a key device, a custom CMOS image sensor realizing a focal-plane pinhole array effect by special pixel addressing and discarding of the unwanted photocarriers was developed. The axial resolution in the confocal mode measured by FWHM for a planar mirror was 8.9 μm, which showed that the confocality has been achieved with the proposed CMOS image sensor.
© 2013 OSA
OCIS Codes
(040.1240) Detectors : Arrays
(180.1790) Microscopy : Confocal microscopy
ToC Category:
Microscopy
History
Original Manuscript: October 31, 2012
Revised Manuscript: December 28, 2012
Manuscript Accepted: January 4, 2013
Published: January 14, 2013
Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics
Citation
Keiichiro Kagawa, Min-Woong Seo, Keita Yasutomi, Susumu Terakawa, and Shoji Kawahito, "Multi-beam confocal microscopy based on a custom image sensor with focal-plane pinhole array effect," Opt. Express 21, 1417-1429 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-2-1417
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References
- H. J. Tiziani and H. M. Uhde, “Three-dimensional analysis by a microlens-array confocal arrangement,” Appl. Opt.33(4), 567–572 (1994). [CrossRef] [PubMed]
- T. Wilson, Confocal Microscopy (Academic Press, 1990).
- J. Pawley and B. Masters, Handbook of Biological Confocal Microscopy, 2nd ed. (Springer, 1995) Chap.11.
- T. Tanaami, S. Otsuki, N. Tomosada, Y. Kosugi, M. Shimizu, and H. Ishida, “High-speed 1-frame/ms scanning confocal microscope with a microlens and Nipkow disks,” Appl. Opt.41(22), 4704–4708 (2002). [CrossRef] [PubMed]
- E. Fossum, “CMOS image sensors – electronic camera on a chip,” IEEE Trans. Electron. Dev.44(10), 1689–1698 (1997). [CrossRef]
- D. Scheffer, B. Dierickx, and G. Meynants, “Random addressable 2048×2048 active pixel sensor,” IEEE Trans. Electron. Dev.44(10), 1716–1720 (1997). [CrossRef]
- J. Ohta, Smart CMOS Image Sensors and Applications (CRC Press, 2007).
- P. Seitz and A. Theuwissen, Single-photon imaging (Springer, 2011).
- H. Yoon, S. Itoh, and S. Kawahito, “A CMOS image sensor with in-pixel two-stage transfer for fluorescence lifetime imaging,” IEEE Trans. Electron. Dev.56(2), 214–221 (2009). [CrossRef]
- D. U. Li, J. Arlt, J. Richardson, R. Walker, A. Buts, D. Stoppa, E. Charbon, and R. Henderson, “Real-time fluorescence lifetime imaging system with a 32 x 32 0.13μm CMOS low dark-count single-photon avalanche diode array,” Opt. Express18(10), 10257–10269 (2010). [CrossRef] [PubMed]
- Z. Li, S. Kawahito, K. Yasutomi, K. Kagawa, J. Ukon, M. Hashimoto, and H. Niioka, “A time-resolved CMOS image sensor with draining-only modulation pixels for fluorescence lifetime imaging,” IEEE Trans. Electron. Dev.59(10), 2715–2722 (2012). [CrossRef]
- P. Lee, R. Gee, R. Guidash, T.-H. Lee, and E. Fossum, “An active pixel sensor fabricated using CMOS/CCD process technology,” Proc. 1995 IEEE Workshop on CCDs and AISs (1995).
- C. Sheppard and D. Shotton, Confocal Laser Scanning Microscopy (Springer, 1997) Chap. 3.
- S. Kawata, O. Nakamura, T. Noda, H. Ooki, K. Ogino, Y. Kuroiwa, and S. Minami, “Laser computed-tomography microscope,” Appl. Opt.29(26), 3805–3809 (1990). [CrossRef] [PubMed]
- K. Yasutomi, S. Itoh, and S. Kawahito, “A two-stage charge transfer active pixel CMOS image sensor with low-noise global shuttering and a dual-shuttering mode,” IEEE Trans. Electron. Dev.58(3), 740–747 (2011). [CrossRef]
- M. Furuta, Y. Nishikawa, T. Inoue, and S. Kawahito, “A high-speed, high-sensitivity digital CMOS image sensor with a global shutter and 12-bit column-parallel cyclic A/D converter,” IEEE J. Solid-state Circuits42(4), 766–774 (2007). [CrossRef]
- K. Kagawa, Y. Ogura, J. Tanida, and Y. Ichioka, “Discrete correlation processor as a building core of a digital optical computing system: architecture and optoelectronic embodiment,” Appl. Opt.38(35), 7276–7281 (1999). [CrossRef] [PubMed]
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