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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 2 — Jan. 28, 2013
  • pp: 1911–1924

Extendable, miniaturized multi-modal optical imaging system: cortical hemodynamic observation in freely moving animals

Rui Liu, Qin Huang, Bing Li, Cui Yin, Chao Jiang, Jia Wang, Jinling Lu, Qingmin Luo, and Pengcheng Li  »View Author Affiliations

Optics Express, Vol. 21, Issue 2, pp. 1911-1924 (2013)

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Observation of brain activities in freely moving animals has become an important approach for neuroscientists to understand the correlation between brain function and behavior. We describe an extendable fiber-optic-based multi-modal imaging system that can concurrently carry out laser speckle contrast imaging (LSCI) of blood flow and optical intrinsic signal (OIS) imaging in freely moving animals, and it could be extended to fluorescence imaging. Our imaging system consists of a multi-source illuminator, a fiber multi-channel optical imaging unit, and a head-mounted microscope. The imaging fiber bundle delivers optical images from the head-mounted microscope to the multi-channel optical imaging unit. Illuminating multi-mode fiber bundles transmit light to the head-mounted microscope which has a mass of less than 1.5 g and includes a gradient index lens, giving the animal maximum movement capability. The internal optical components are adjustable, allowing for a change in magnification and field of view. We test the system by observing hemodynamic changes during cortical spreading depression (CSD) in freely moving and anesthetized animals by simultaneous LSCI and dual-wavelength OIS imaging. Hemodynamic parameters were calculated. Significant differences in CSD propagation durations between the two states were observed. Furthermore, it is capable of performing fluorescence imaging to explore animal behavior and the underlying brain functional activity further.

© 2013 OSA

OCIS Codes
(110.2350) Imaging systems : Fiber optics imaging
(110.2960) Imaging systems : Image analysis
(170.0180) Medical optics and biotechnology : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5380) Medical optics and biotechnology : Physiology
(170.6480) Medical optics and biotechnology : Spectroscopy, speckle

ToC Category:
Imaging Systems

Original Manuscript: November 8, 2012
Revised Manuscript: December 29, 2012
Manuscript Accepted: January 8, 2013
Published: January 17, 2013

Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics

Rui Liu, Qin Huang, Bing Li, Cui Yin, Chao Jiang, Jia Wang, Jinling Lu, Qingmin Luo, and Pengcheng Li, "Extendable, miniaturized multi-modal optical imaging system: cortical hemodynamic observation in freely moving animals," Opt. Express 21, 1911-1924 (2013)

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