OSA's Digital Library

Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 20 — Oct. 7, 2013
  • pp: 23921–23926

A fast fluorescence imaging flow cytometer for phytoplankton analysis

Jianglai Wu and Robert K. Y. Chan  »View Author Affiliations

Optics Express, Vol. 21, Issue 20, pp. 23921-23926 (2013)

View Full Text Article

Enhanced HTML    Acrobat PDF (1114 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We report a fast fluorescence imaging flow cytometer for phytoplankton analysis that can achieve a volume flow rate up to 1ml/min. The instrument shows a high immunity to motion blur in image captured with a lateral resolution of 0.75 ± 0.06 μm for a wide size range ~1 μm to ~200 μm. This is made possible by suppressing the out-of-focus light using thin light sheet illumination and image deconvolution, and by precluding the motion-blur with a unique flow configuration. Preliminary results from untreated coastal water samples show the technique has high potential as a practical field instrument for monitoring phytoplankton abundance and species composition.

© 2013 Optical Society of America

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(100.1830) Image processing : Deconvolution
(110.2970) Imaging systems : Image detection systems
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Atmospheric and Oceanic Optics

Original Manuscript: July 26, 2013
Revised Manuscript: September 19, 2013
Manuscript Accepted: September 22, 2013
Published: September 30, 2013

Jianglai Wu and Robert K. Y. Chan, "A fast fluorescence imaging flow cytometer for phytoplankton analysis," Opt. Express 21, 23921-23926 (2013)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. Z. V. Finkel, J. Beardall, K. J. Flynn, A. Quigg, T. A. V. Rees, and J. A. Raven, “Phytoplankton in a changing world: cell size and elemental stoichiometry,” J. Plankton Res.32(1), 119–137 (2010). [CrossRef]
  2. J. C. H. Peeters, G. B. J. Dubelaar, J. Ringelberg, and J. W. M. Visser, “Optical plankton analyser: a flow cytometer for plankton analysis, I: design considerations,” Cytometry10(5), 522–528 (1989). [CrossRef] [PubMed]
  3. M. F. Wilkins, L. Boddy, C. W. Morris, and R. R. Jonker, “Identification of phytoplankton from flow cytometry data by using radial basis function neural networks,” Appl. Environ. Microbiol.65(10), 4404–4410 (1999). [PubMed]
  4. J. Picot, C. L. Guerin, C. Le Van Kim, and C. M. Boulanger, “Flow cytometry: retrospective, fundamentals and recent instrumentation,” Cytotechnology64(2), 109–130 (2012). [CrossRef] [PubMed]
  5. V. Kachel, G. Benker, K. Lichtnau, G. Valet, and E. Glossner, “Fast imaging in flow: a means of combining flow-cytometry and image analysis,” J. Histochem. Cytochem.27(1), 335–341 (1979). [CrossRef] [PubMed]
  6. V. Kachel, G. Benker, W. Weiss, E. Glossner, G. Valet, and O. Ahrens, “Problems of fast imaging in flow,” Flow CytometryIV, 45–49 (1980).
  7. K. Goda, K. K. Tsia, and B. Jalali, “Serial time-encoded amplified imaging for real-time observation of fast dynamic phenomena,” Nature458(7242), 1145–1149 (2009). [CrossRef] [PubMed]
  8. D. A. Basiji and W. E. Ortyn, “Imaging and analyzing parameters of small moving objects such as cells,” Amnis Corporation, assignee. US Patent 6211955, 2000–03–29 (2001).
  9. S. S. Gorthi, D. Schaak, and E. Schonbrun, “Fluorescence imaging of flowing cells using a temporally coded excitation,” Opt. Express21(4), 5164–5170 (2013). [CrossRef] [PubMed]
  10. R. J. Olson and H. M. Sosik, “A submersible imaging-in-flow instrument to analyze nano and microplankton: Imaging FlowCytobot,” Limnol. Oceanogr. Methods5, 195–203 (2007). [CrossRef]
  11. W. E. Ortyn, D. J. Perry, V. Venkatachalam, L. Liang, B. E. Hall, K. Frost, and D. A. Basiji, “Extended depth of field Imaging for high speed cell analysis,” Cytometry A71(4), 215–231 (2007). [CrossRef] [PubMed]
  12. C. K. Sieracki, M. E. Sieracki, and C. S. Yentsch, “An imaging-in-flow system for automated analysis of marine microplankton,” Mar. Ecol. Prog. Ser.168, 285–296 (1998). [CrossRef]
  13. V. Kachel and J. Wietzorrek, “Flow cytometry and integrated imaging,” Sci. Mar.64(2), 247–254 (2000).
  14. B. K. McKenna, J. G. Evans, M. C. Cheung, and D. J. Ehrlich, “A parallel microfluidic flow cytometer for high-content screening,” Nat. Methods8(5), 401–403 (2011). [CrossRef] [PubMed]
  15. J. Wu, J. Li, and R. K. Y. Chan, “A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis,” Opt. Express21(12), 14474–14480 (2013). [CrossRef] [PubMed]
  16. K. Greger, J. Swoger, and E. H. K. Stelzer, “Basic building units and properties of a fluorescence single plane illumination microscope,” Rev. Sci. Instrum.78(2), 023705 (2007). [CrossRef] [PubMed]
  17. E. Fuchs, J. S. Jaffe, R. A. Long, and F. Azam, “Thin laser light sheet microscope for microbial oceanography,” Opt. Express10(2), 145–154 (2002). [CrossRef] [PubMed]
  18. J. A. Conchello and M. E. Dresser, “Extended depth-of-focus microscopy via constrained deconvolution,” J. Biomed. Opt.12(6), 064026 (2007). [CrossRef] [PubMed]
  19. Hong Kong Environmental Protection Department, “Marine Water Quality Reports,” (2013). http://www.epd.gov.hk/epd/english/environmentinhk/water/marine_quality/mwq_report.html .

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4 Fig. 5

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited