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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 20 — Oct. 7, 2013
  • pp: 23921–23926

A fast fluorescence imaging flow cytometer for phytoplankton analysis

Jianglai Wu and Robert K. Y. Chan  »View Author Affiliations


Optics Express, Vol. 21, Issue 20, pp. 23921-23926 (2013)
http://dx.doi.org/10.1364/OE.21.023921


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Abstract

We report a fast fluorescence imaging flow cytometer for phytoplankton analysis that can achieve a volume flow rate up to 1ml/min. The instrument shows a high immunity to motion blur in image captured with a lateral resolution of 0.75 ± 0.06 μm for a wide size range ~1 μm to ~200 μm. This is made possible by suppressing the out-of-focus light using thin light sheet illumination and image deconvolution, and by precluding the motion-blur with a unique flow configuration. Preliminary results from untreated coastal water samples show the technique has high potential as a practical field instrument for monitoring phytoplankton abundance and species composition.

© 2013 Optical Society of America

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(100.1830) Image processing : Deconvolution
(110.2970) Imaging systems : Image detection systems
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Atmospheric and Oceanic Optics

History
Original Manuscript: July 26, 2013
Revised Manuscript: September 19, 2013
Manuscript Accepted: September 22, 2013
Published: September 30, 2013

Citation
Jianglai Wu and Robert K. Y. Chan, "A fast fluorescence imaging flow cytometer for phytoplankton analysis," Opt. Express 21, 23921-23926 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-20-23921


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