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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 20 — Oct. 7, 2013
  • pp: 23985–23996

Imaging through scattering microfluidic channels by digital holography for information recovery in lab on chip

V. Bianco, M. Paturzo, O. Gennari, A. Finizio, and P. Ferraro  »View Author Affiliations


Optics Express, Vol. 21, Issue 20, pp. 23985-23996 (2013)
http://dx.doi.org/10.1364/OE.21.023985


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Abstract

We tackle the problem of information recovery and imaging through scattering microfluidic chips by means of digital holography (DH). In many cases the chip can become opalescent due to residual deposits settling down the inner channel faces, biofilm formation, scattering particle uptake by the channel cladding or its damaging by corrosive substances, or even by condensing effect on the exterior channels walls. In these cases white-light imaging is severely degraded and no information is obtainable at all about the flowing samples. Here we investigate the problem of counting and estimating velocity of cells flowing inside a scattering chip. Moreover we propose and test a method based on the recording of multiple digital holograms to retrieve improved phase-contrast images despite the strong scattering effect. This method helps, thanks to DH, to recover information which, otherwise, would be completely lost.

© 2013 OSA

OCIS Codes
(180.0180) Microscopy : Microscopy
(290.0290) Scattering : Scattering
(110.0113) Imaging systems : Imaging through turbid media
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: June 27, 2013
Revised Manuscript: July 30, 2013
Manuscript Accepted: August 3, 2013
Published: October 1, 2013

Citation
V. Bianco, M. Paturzo, O. Gennari, A. Finizio, and P. Ferraro, "Imaging through scattering microfluidic channels by digital holography for information recovery in lab on chip," Opt. Express 21, 23985-23996 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-20-23985


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