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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 20 — Oct. 7, 2013
  • pp: 24210–24218

Reflection-mode optical-resolution photoacoustic microscopy based on a reflective objective

Hui Wang, Xiaoquan Yang, Yanyan Liu, Bowen Jiang, and Qingming Luo  »View Author Affiliations


Optics Express, Vol. 21, Issue 20, pp. 24210-24218 (2013)
http://dx.doi.org/10.1364/OE.21.024210


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Abstract

We developed a new reflection-mode optical-resolution photoacoustic microscopy (OR-PAM) based on the cooperation of a reflective objective and an ultrasonic transducer. The reflective objective is used to produce nearly diffraction-limited optical focusing, and the excited ultrasound waves are then directly detected by an ultrasonic transducer that was placed in the central cone of the objective. This new design avoids the coupling between optical focusing and ultrasound transmission in the reflection mode. Moreover, the proposed system is able to provide lateral resolution of 1.2 μm at 580 nm, penetration depth of 0.9 mm in biological tissues, and a work distance of 6.0 mm. We present in vivo imaging of the microvasculature in mouse ears and in vitro imaging of red blood cells (RBCs), which demonstrate the capability of the system to study microcirculation.

© 2013 Optical Society of America

OCIS Codes
(110.5120) Imaging systems : Photoacoustic imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: August 2, 2013
Revised Manuscript: September 9, 2013
Manuscript Accepted: September 24, 2013
Published: October 2, 2013

Citation
Hui Wang, Xiaoquan Yang, Yanyan Liu, Bowen Jiang, and Qingming Luo, "Reflection-mode optical-resolution photoacoustic microscopy based on a reflective objective," Opt. Express 21, 24210-24218 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-20-24210


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