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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 21 — Oct. 21, 2013
  • pp: 24819–24828

Analysis of cellular objects through diffraction images acquired by flow cytometry

Jun Zhang, Yuanming Feng, Marina S. Moran, Jun Q. Lu, Li V. Yang, Yu Sa, Ning Zhang, Lixue Dong, and Xin-Hua Hu  »View Author Affiliations


Optics Express, Vol. 21, Issue 21, pp. 24819-24828 (2013)
http://dx.doi.org/10.1364/OE.21.024819


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Abstract

It was found that the diffraction images acquired along the side scattering directions with objects in a cell sample contain pattern variations at both the global and local scales. We show here that the global pattern variation is associated with the categorical size and morphological heterogeneity of the imaged objects. An automated image processing method has been developed to separate the acquired diffraction images into three types of global patterns. Combined with previously developed method for quantifying local texture pattern variations, the new method allows fully automated analysis of diffraction images for rapid and label-free classification of cells according to their 3D morphology.

© 2013 Optical Society of America

OCIS Codes
(110.1650) Imaging systems : Coherence imaging
(170.1530) Medical optics and biotechnology : Cell analysis
(290.5850) Scattering : Scattering, particles

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: August 29, 2013
Revised Manuscript: September 26, 2013
Manuscript Accepted: September 26, 2013
Published: October 9, 2013

Citation
Jun Zhang, Yuanming Feng, Marina S. Moran, Jun Q. Lu, Li V. Yang, Yu Sa, Ning Zhang, Lixue Dong, and Xin-Hua Hu, "Analysis of cellular objects through diffraction images acquired by flow cytometry," Opt. Express 21, 24819-24828 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-21-24819


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References

  1. A. K. Dunn, C. L. Smithpeter, A. J. Welch, and R. R. Richards-Kortum, “Finite-difference time-domain simulation of light scattering from single cells,” J. Biomed. Opt.2(3), 262–266 (1997). [CrossRef] [PubMed]
  2. J. R. Mourant, M. Canpolat, C. Brocker, O. Esponda-Ramos, T. M. Johnson, A. Matanock, K. Stetter, and J. P. Freyer, “Light scattering from cells: the contribution of the nucleus and the effects of proliferative status,” J. Biomed. Opt.5(2), 131–137 (2000). [CrossRef] [PubMed]
  3. J. Q. Lu, P. Yang, and X. H. Hu, “SSimulations of light scattering from a biconcave red blood cell using the finite-difference time-domain method,” J. Biomed. Opt.10, 024022 (2005). [CrossRef] [PubMed]
  4. R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, “Effect of detailed cell structure on light scattering distribution: FDTD study of a B-cell with 3D structure constructed from confocal images,” J. Quant. Spectrosc. Radiat. Transf.102(1), 25–36 (2006). [CrossRef]
  5. H. Ding, J. Q. Lu, R. S. Brock, T. J. McConnell, J. F. Ojeda, K. M. Jacobs, and X. H. Hu, “Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850 nm,” J. Biomed. Opt.12(3), 034032 (2007). [CrossRef] [PubMed]
  6. M. R. Melamed, T. Lindmo, and M. L. Mendelsohn, Flow Cytometry and Sorting (Wiley-Liss, 1990).
  7. A. N. Shvalov, I. V. Surovtsev, A. V. Chernyshev, J. T. Soini, and V. P. Maltsev, “Particle classification from light scattering with the scanning flow cytometer,” Cytometry37(3), 215–220 (1999). [CrossRef] [PubMed]
  8. D. I. Strokotov, A. E. Moskalensky, V. M. Nekrasov, and V. P. Maltsev, “Polarized light-scattering profile-advanced characterization of nonspherical particles with scanning flow cytometry,” Cytometry A79(7), 570–579 (2011). [CrossRef] [PubMed]
  9. S. Holler, Y. Pan, R. K. Chang, J. R. Bottiger, S. C. Hill, and D. B. Hillis, “Two-dimensional angular optical scattering for the characterization of airborne microparticles,” Opt. Lett.23(18), 1489–1491 (1998). [CrossRef] [PubMed]
  10. J. Neukammer, C. Gohlke, A. Höpe, T. Wessel, and H. Rinneberg, “Angular distribution of light scattered by single biological cells and oriented particle agglomerates,” Appl. Opt.42(31), 6388–6397 (2003). [CrossRef] [PubMed]
  11. K. Singh, C. Liu, C. Capjack, W. Rozmus, and C. J. Backhouse, “Analysis of cellular structure by light scattering measurements in a new cytometer design based on a liquid-core waveguide,” IEE proceedings 151, 10–16 (2004).
  12. X. T. Su, K. Singh, C. Capjack, J. Petrácek, C. Backhouse, and W. Rozmus, “Measurements of light scattering in an integrated microfluidic waveguide cytometer,” J. Biomed. Opt.13(2), 024024 (2008). [CrossRef] [PubMed]
  13. X. Su, S. E. Kirkwood, M. Gupta, L. Marquez-Curtis, Y. Qiu, A. Janowska-Wieczorek, W. Rozmus, and Y. Y. Tsui, “Microscope-based label-free microfluidic cytometry,” Opt. Express19(1), 387–398 (2011). [CrossRef] [PubMed]
  14. X. Su, Y. Qiu, L. Marquez-Curtis, M. Gupta, C. E. Capjack, W. Rozmus, A. Janowska-Wieczorek, and Y. Y. Tsui, “Label-free and noninvasive optical detection of the distribution of nanometer-size mitochondria in single cells,” J. Biomed. Opt.16(6), 067003 (2011). [CrossRef] [PubMed]
  15. K. M. Jacobs, J. Q. Lu, and X. H. Hu, “Development of a diffraction imaging flow cytometer,” Opt. Lett.34(19), 2985–2987 (2009). [CrossRef] [PubMed]
  16. K. M. Jacobs, L. V. Yang, J. Ding, A. E. Ekpenyong, R. Castellone, J. Q. Lu, and X. H. Hu, “Diffraction imaging of spheres and melanoma cells with a microscope objective,” J Biophotonics2(8-9), 521–527 (2009). [CrossRef] [PubMed]
  17. K. Dong, Y. Feng, K. M. Jacobs, J. Q. Lu, R. S. Brock, L. V. Yang, F. E. Bertrand, M. A. Farwell, and X. H. Hu, “Label-free classification of cultured cells through diffraction imaging,” Biomed. Opt. Express2(6), 1717–1726 (2011). [CrossRef] [PubMed]
  18. S. Yu, J. Zhang, M. S. Moran, J. Q. Lu, Y. Feng, and X. H. Hu, “A novel method of diffraction imaging flow cytometry for sizing microspheres,” Opt. Express20(20), 22245–22251 (2012). [CrossRef] [PubMed]
  19. Y. Sa, Y. Feng, K. M. Jacobs, J. Yang, R. Pan, I. Gkigkitzis, J. Q. Lu, and X. H. Hu, “Study of low speed flow cytometry for diffraction imaging with different chamber and nozzle designs,” Cytometry A (to be published). [PubMed]
  20. Y. Feng, N. Zhang, K. M. Jacobs, W. Jiang, L. V. Yang, Z. Li, J. Zhang, J. Q. Lu, and X. H. Hu, “Classification of Jurkat T and Ramos B cells,” Opt. Lett.submitted.
  21. R. D. Castellone, N. R. Leffler, L. Dong, and L. V. Yang, “Inhibition of tumor cell migration and metastasis by the proton-sensing GPR4 receptor,” Cancer Lett.312(2), 197–208 (2011). [CrossRef] [PubMed]
  22. M. Born and E. Wolf, Principles of Optics (Cambridge University Press, 1999).
  23. C. Cortes and V. Vapnik, “Support-vector networks,” Mach. Learn.20(3), 273–297 (1995). [CrossRef]
  24. C. C. Chang and C. J. Lin, “LIBSVM: a library for support vector machines,” (2001), http://www.csie.ntu.edu.tw/~cjlin/libsvm .
  25. A. G. Hoekstra, M. D. Grimminck, and P. M. A. Sloot, “Large Scale Simulations of Elastic Light Scattering by a Fast Discrete Dipole Approximation,” Int. J. Mod. Phys. C9(01), 87–102 (1998). [CrossRef]
  26. M. A. Yurkin and A. G. Hoekstra, “The discrete dipole approximation: an overview and recent developments,” J. Quant. Spectrosc. Radiat. Transf.106(1-3), 558–589 (2007). [CrossRef]
  27. M. A. Yurkin, A. G. Hoekstra, R. S. Brock, and J. Q. Lu, “Systematic comparison of the discrete dipole approximation and the finite difference time domain method for large dielectric scatterers,” Opt. Express15(26), 17902–17911 (2007). [CrossRef] [PubMed]
  28. Y. Zhang, Y. Feng, C. R. Justus, W. Jiang, Z. Li, J. Q. Lu, R. S. Brock, M. K. McPeek, D. A. Weidner, L. V. Yang, and X. H. Hu, “Comparative study of 3D morphology and functions on genetically engineered mouse melanoma cells,” Integr Biol (Camb)4(11), 1428–1436 (2012). [CrossRef] [PubMed]
  29. M. Moran, Correlating the Morphological and Light Scattering Properties of Biological Cells, Ph.D. Dissertation (East Carolina University, 2013).
  30. N. Kanopoulos, N. Vasanthavada, and R. L. Baker, “Design of an image edge detection filter using the Sobel operator,” IEEE J. Solid-State Circuits23(2), 358–367 (1988). [CrossRef]
  31. T. Kanungo, D. M. Mount, N. S. Netanyahu, C. D. Piatko, R. Silverman, and A. Y. Wu, “An efficient k-means clustering algorithm: analysis and implementation,” IEEE Trans. Pattern Anal. Mach. Intell.24(7), 881–892 (2002). [CrossRef]

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