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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 21 — Oct. 21, 2013
  • pp: 25346–25355

Image-inspired 3D multiphoton excited fabrication of extracellular matrix structures by modulated raster scanning

Visar Ajeti, Chi-Hsiang Lien, Shean-Jen Chen, Ping-Jung Su, Jayne M. Squirrell, Katharine H. Molinarolo, Gary E. Lyons, Kevin W. Eliceiri, Brenda M. Ogle, and Paul J. Campagnola  »View Author Affiliations

Optics Express, Vol. 21, Issue 21, pp. 25346-25355 (2013)

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Multiphoton excited photochemistry is a powerful 3D fabrication tool that produces sub-micron feature sizes. Here we exploit the freeform nature of the process to create models of the extracellular matrix (ECM) of several tissues, where the design blueprint is derived directly from high resolution optical microscopy images (e.g. fluorescence and Second Harmonic Generation). To achieve this goal, we implemented a new form of instrument control, termed modulated raster scanning, where rapid laser shuttering (10 MHz) is used to directly map the greyscale image data to the resulting protein concentration in the fabricated scaffold. Fidelity in terms of area coverage and relative concentration relative to the image data is ~95%. We compare the results to an STL approach, and find the new scheme provides significantly improved performance. We suggest the method will enable a variety of cell-matrix studies in cancer biology and also provide insight into generating scaffolds for tissue engineering.

© 2013 Optical Society of America

OCIS Codes
(120.4610) Instrumentation, measurement, and metrology : Optical fabrication
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(220.4000) Optical design and fabrication : Microstructure fabrication
(350.3450) Other areas of optics : Laser-induced chemistry

ToC Category:
Laser Microfabrication

Original Manuscript: July 29, 2013
Revised Manuscript: September 19, 2013
Manuscript Accepted: September 22, 2013
Published: October 17, 2013

Visar Ajeti, Chi-Hsiang Lien, Shean-Jen Chen, Ping-Jung Su, Jayne M. Squirrell, Katharine H. Molinarolo, Gary E. Lyons, Kevin W. Eliceiri, Brenda M. Ogle, and Paul J. Campagnola, "Image-inspired 3D multiphoton excited fabrication of extracellular matrix structures by modulated raster scanning," Opt. Express 21, 25346-25355 (2013)

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