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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 22 — Nov. 4, 2013
  • pp: 25632–25642

High resolution imaging with differential infrared absorption micro-spectroscopy

Isabel Pita, Nordine Hendaoui, Ning Liu, Mahendar Kumbham, Syed A. M. Tofail, André Peremans, and Christophe Silien  »View Author Affiliations

Optics Express, Vol. 21, Issue 22, pp. 25632-25642 (2013)

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Although confocal infrared (IR) absorption micro-spectroscopy is well established for far-field chemical imaging, its scope remains restricted since diffraction limits the spatial resolution to values a little above half the radiation wavelength. Yet, the successful implementations of below-the-diffraction limit far-field fluorescence microscopies using saturated irradiation patterns for example for stimulated-emission depletion and saturated structured-illumination suggest the possibility of using a similar optical patterning strategy for infrared absorption mapping at high resolution. Simulations are used to show that the simple mapping of the difference in transmitted/reflected IR energy between a saturated vortex-shaped beam and a Gaussian reference with a confocal microscope affords the generation of high-resolution vibrational absorption images. On the basis of experimentally relevant parameters, the simulations of the differential absorption scheme reveal a spatial resolution better than a tenth of the wavelength for incident energies about a decade above the saturation threshold. The saturated structured illumination concepts are thus expected to be compatible with the establishment of point-like point-spread functions for measuring the absorbance of samples with a scanning confocal microscope recording the differential transmission/reflection.

© 2013 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(300.1030) Spectroscopy : Absorption
(300.6340) Spectroscopy : Spectroscopy, infrared
(300.6360) Spectroscopy : Spectroscopy, laser
(300.6390) Spectroscopy : Spectroscopy, molecular

ToC Category:

Original Manuscript: May 22, 2013
Revised Manuscript: July 8, 2013
Manuscript Accepted: July 16, 2013
Published: October 21, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Isabel Pita, Nordine Hendaoui, Ning Liu, Mahendar Kumbham, Syed A. M. Tofail, André Peremans, and Christophe Silien, "High resolution imaging with differential infrared absorption micro-spectroscopy," Opt. Express 21, 25632-25642 (2013)

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