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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 22 — Nov. 4, 2013
  • pp: 27023–27031

Dengue immunoassay with an LSPR fiber optic sensor

Alexandre R. Camara, Paula M. P. Gouvêa, Ana Carolina M. S. Dias, Arthur M. B. Braga, Rosa F. Dutra, Renato E. de Araujo, and Isabel C. S. Carvalho  »View Author Affiliations


Optics Express, Vol. 21, Issue 22, pp. 27023-27031 (2013)
http://dx.doi.org/10.1364/OE.21.027023


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Abstract

Dengue fever is a viral disease that affects millions of people worldwide. Specific tests for dengue are not usually performed due to high costs, complicated procedures and, in some cases, long time to yield a result. For widespread use of specific tests to be possible, fast, reliable and fairly simple methods are needed. In this paper, we present a new dengue diagnostic method for the acute phase of the infection. The method proposed uses an all-optical fiber sensor based on Localized Surface Plasmon Resonance (LSPR) and specular reflection from gold nanoparticles (AuNPs). Dengue anti-NS1 antibody was immobilized on AuNPs deposited on the endface of a standard multimode fiber (62.5µm/125µm). The sensor is able to detect NS1 antigen at different concentrations, with limit of quantification estimated to be 0.074 μg/ml = 1.54 nM. These results indicate that the sensor could potentially be used for dengue diagnosis in the acute phase of the infection.

© 2013 Optical Society of America

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(240.6680) Optics at surfaces : Surface plasmons
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:
Sensors

History
Original Manuscript: August 20, 2013
Revised Manuscript: October 2, 2013
Manuscript Accepted: October 7, 2013
Published: October 31, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Alexandre R. Camara, Paula M. P. Gouvêa, Ana Carolina M. S. Dias, Arthur M. B. Braga, Rosa F. Dutra, Renato E. de Araujo, and Isabel C. S. Carvalho, "Dengue immunoassay with an LSPR fiber optic sensor," Opt. Express 21, 27023-27031 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-22-27023


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