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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 22 — Nov. 4, 2013
  • pp: 27338–27343

Gold nanoparticles for enhanced single molecule fluorescence analysis at micromolar concentration

Deep Punj, Juan de Torres, Hervé Rigneault, and Jérôme Wenger  »View Author Affiliations

Optics Express, Vol. 21, Issue 22, pp. 27338-27343 (2013)

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Individual metal nanoparticles represent an inexpensive and versatile platform to enhance the detection of fluorescent species at biologically relevant concentrations. Here we use fluorescence correlation spectroscopy to quantify the near-field detection volume and average fluorescence enhancement factors set by a single gold nanoparticle. We demonstrate detection volumes down to 270 zeptoliters (three orders of magnitude beyond the diffraction barrier) together with 60-fold enhancement of the fluorescence brightness per molecule.

© 2013 OSA

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(240.0240) Optics at surfaces : Optics at surfaces
(350.4238) Other areas of optics : Nanophotonics and photonic crystals

ToC Category:

Original Manuscript: July 24, 2013
Revised Manuscript: September 12, 2013
Manuscript Accepted: September 12, 2013
Published: November 4, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics
Surface Plasmon Photonics (2013) Optics Express

Deep Punj, Juan de Torres, Hervé Rigneault, and Jérôme Wenger, "Gold nanoparticles for enhanced single molecule fluorescence analysis at micromolar concentration," Opt. Express 21, 27338-27343 (2013)

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