OSA's Digital Library

Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 23 — Nov. 18, 2013
  • pp: 28062–28071

Portable spherical array probe for volumetric real-time optoacoustic imaging at centimeter-scale depths

X. Luís Deán-Ben and Daniel Razansky  »View Author Affiliations

Optics Express, Vol. 21, Issue 23, pp. 28062-28071 (2013)

View Full Text Article

Enhanced HTML    Acrobat PDF (2308 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We report on a novel hand-held imaging probe for real-time optoacoustic visualization of deep tissues in three dimensions. The system incorporates an annular two-dimensional array of ultrasonic sensors densely distributed on a spherical surface. Simultaneous recording and processing of time-resolved data from all the channels enables acquisition of entire volumetric data sets for each illumination laser pulse. The proposed solution utilizes a transparent membrane in order to allow efficient coupling of optoacoustically generated waves to the ultrasonic detectors while avoiding direct contact of the imaged object with the coupling medium. The hand-held approach further allows convenient handling of both pre-clinical experiments as well as clinical measurements in human subjects. Here we demonstrate an imaging speed of 10 volumetric frames per second with spatial resolution down to 200 micrometers in the imaged region while also achieving imaging depth of more than 1.5 cm in living tissues without signal averaging.

© 2013 Optical Society of America

OCIS Codes
(110.5120) Imaging systems : Photoacoustic imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
Imaging Systems

Original Manuscript: June 5, 2013
Revised Manuscript: July 11, 2013
Manuscript Accepted: July 11, 2013
Published: November 8, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

X. Luís Deán-Ben and Daniel Razansky, "Portable spherical array probe for volumetric real-time optoacoustic imaging at centimeter-scale depths," Opt. Express 21, 28062-28071 (2013)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. D. Razansky, N. C. Deliolanis, C. Vinegoni, and V. Ntziachristos, “Deep tissue optical and optoacoustic molecular imaging technologies for pre-clinical research and drug discovery,” Curr. Pharm. Biotechnol.13(4), 504–522 (2012). [CrossRef] [PubMed]
  2. M. A. Pysz, S. S. Gambhir, and J. K. Willmann, “Molecular imaging: current status and emerging strategies,” Clin. Radiol.65(7), 500–516 (2010). [CrossRef] [PubMed]
  3. F. Leblond, S. C. Davis, P. A. Valdés, and B. W. Pogue, “Pre-clinical whole-body fluorescence imaging: Review of instruments, methods and applications,” J. Photochem. Photobiol. B98(1), 77–94 (2010). [CrossRef] [PubMed]
  4. C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods5(1), 45–47 (2007). [CrossRef] [PubMed]
  5. L. V. Wang and H.-I. Wu, Biomedical optics: principles and imaging (Wiley, 2007).
  6. L. V. Wang and S. Hu, “Photoacoustic tomography: in vivo imaging from organelles to organs,” Science335(6075), 1458–1462 (2012). [CrossRef] [PubMed]
  7. P. Beard, “Biomedical photoacoustic imaging,” Interface Focus1(4), 602–631 (2011). [CrossRef] [PubMed]
  8. D. Razansky, “Multi-spectral optoacoustic tomography – volumetric color hearing in real time,” IEEE J. Sel. Top. Quantum Electron.18(3), 1234–1243 (2012). [CrossRef]
  9. E. Herzog, A. Taruttis, N. Beziere, A. A. Lutich, D. Razansky, and V. Ntziachristos, “Optical imaging of cancer heterogeneity with multispectral optoacoustic tomography,” Radiology263(2), 461–468 (2012). [CrossRef] [PubMed]
  10. J. J. Yao, J. Xia, K. I. Maslov, M. Nasiriavanaki, V. Tsytsarev, A. V. Demchenko, and L. V. Wang, “Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivo,” Neuroimage64, 257–266 (2013). [CrossRef] [PubMed]
  11. L. Tong, Q. S. Wei, A. Wei, and J. X. Cheng, “Gold nanorods as contrast agents for biological imaging: optical properties, surface conjugation and photothermal effects,” Photochem. Photobiol.85(1), 21–32 (2009). [CrossRef] [PubMed]
  12. A. Buehler, E. Herzog, A. Ale, B. A. Smith, V. Ntziachristos, and D. Razansky, “High resolution targeting of tumor apoptosis in living mice by means of multispectral optoacoustic tomography,” Eur. J. Nucl. Med. Mol. Imag. Res.2, 14 (2012).
  13. J. Laufer, P. Johnson, E. Zhang, B. Treeby, B. Cox, B. Pedley, and P. Beard, “In vivo preclinical photoacoustic imaging of tumor vasculature development and therapy,” J. Biomed. Opt.17(5), 056016 (2012). [CrossRef] [PubMed]
  14. S. P. Johnson, J. G. Laufer, E. Z. Zhang, P. C. Beard, and R. B. Pedley, “Determination of differential tumour vascular pathophysiology in vivo by photoacoustic imaging,” Eur. J. Cancer48, S186–S187 (2012). [CrossRef]
  15. R. Ma, M. Distel, X. L. Deán-Ben, V. Ntziachristos, and D. Razansky, “Non-invasive whole-body imaging of adult zebrafish with optoacoustic tomography,” Phys. Med. Biol.57(22), 7227–7237 (2012). [CrossRef] [PubMed]
  16. B. Xia, D. Piras, J. C. G. van Hespen, W. Steenbergen, and S. Manohar, “A new acoustic lens material for large area detectors in photoacoustic breast tomography,” Photoacoustics1(2), 9–18 (2013). [CrossRef]
  17. J. M. Yang, C. Favazza, R. Chen, J. Yao, X. Cai, K. Maslov, Q. Zhou, K. K. Shung, and L. V. Wang, “Simultaneous functional photoacoustic and ultrasonic endoscopy of internal organs in vivo,” Nat. Med.18(8), 1297–1302 (2012). [CrossRef] [PubMed]
  18. D. Razansky, N. J. Harlaar, J. L. Hillebrands, A. Taruttis, E. Herzog, C. J. Zeebregts, G. M. van Dam, and V. Ntziachristos, “Multispectral optoacoustic tomography of matrix metalloproteinase activity in vulnerable human carotid plaques,” Mol. Imaging Biol.14(3), 277–285 (2012). [CrossRef] [PubMed]
  19. Y. Xu, L. V. Wang, G. Ambartsoumian, and P. Kuchment, “Reconstructions in limited-view thermoacoustic tomography,” Med. Phys.31(4), 724–733 (2004). [CrossRef] [PubMed]
  20. R. A. Kruger, R. B. Lam, D. R. Reinecke, S. P. Del Rio, and R. P. Doyle, “Photoacoustic angiography of the breast,” Med. Phys.37(11), 6096–6100 (2010). [CrossRef] [PubMed]
  21. D. Van de Sompel, L. S. Sasportas, A. Dragulescu-Andrasi, S. Bohndiek, and S. S. Gambhir, “Improving image quality by accounting for changes in water temperature during a photoacoustic tomography scan,” PLoS ONE7(10), e45337 (2012). [CrossRef] [PubMed]
  22. H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, “Whole-body three-dimensional optoacoustic tomography system for small animals,” J. Biomed. Opt.14(6), 064007 (2009). [CrossRef] [PubMed]
  23. D. R. Reinecke, R. A. Kruger, R. B. Lam, and S. P. Del Rio, “Co-registered photoacoustic, thermoacoustic and ultrasound mouse imaging,” Proc. SPIE7564, 756420 (2010). [CrossRef]
  24. A. Buehler, X. L. Deán-Ben, J. Claussen, V. Ntziachristos, and D. Razansky, “Three-dimensional optoacoustic tomography at video rate,” Opt. Express20(20), 22712–22719 (2012). [CrossRef] [PubMed]
  25. M. P. Fronheiser, S. A. Ermilov, H. P. Brecht, A. Conjusteau, R. Su, K. Mehta, and A. A. Oraevsky, “Real-time optoacoustic monitoring and three-dimensional mapping of a human arm vasculature,” J. Biomed. Opt.15(2), 021305 (2010). [CrossRef] [PubMed]
  26. C. Kim, T. N. Erpelding, L. Jankovic, M. D. Pashley, and L. V. Wang, “Deeply penetrating in vivo photoacoustic imaging using a clinical ultrasound array system,” Biomed. Opt. Express1(1), 278–284 (2010). [CrossRef] [PubMed]
  27. P. D. Kumavor, C. Xu, A. Aguirre, J. Gamelin, Y. Ardeshirpour, B. Tavakoli, S. Zanganeh, U. Alqasemi, Y. Yang, and Q. Zhu, “Target detection and quantification using a hybrid hand-held diffuse optical tomography and photoacoustic tomography system,” J. Biomed. Opt.16(4), 046010 (2011). [CrossRef] [PubMed]
  28. D. Queiros, X. L. Dean-Ben, A. Buehler, D. Razansky, A. Rosenthal, and V. Ntziachristos, “Modeling the shape of cylindrically focused transducers in three-dimensional optoacoustic tomography,” J. Biomed. Opt.18(7) 076014 (2013).
  29. A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, “Multispectral optoacoustic tomography of myocardial infarction,” Photoacoustics1(1), 3–8 (2013). [CrossRef]
  30. X. L. Deán-Ben, A. Buehler, V. Ntziachristos, and D. Razansky, “Accurate model-based reconstruction algorithm for three-dimensional optoacoustic tomography,” IEEE Trans. Med. Imaging31(10), 1922–1928 (2012). [CrossRef] [PubMed]
  31. A. Rosenthal, V. Ntziachristos, and D. Razansky, “Model-based optoacoustic inversion with arbitrary-shape detectors,” Med. Phys.38(7), 4285–4295 (2011). [CrossRef] [PubMed]
  32. X. L. Deán-Ben, D. Razansky, and V. Ntziachristos, “The effects of acoustic attenuation in optoacoustic signals,” Phys. Med. Biol.56(18), 6129–6148 (2011). [CrossRef] [PubMed]
  33. A. Rosenthal, V. Ntziachristos, and D. Razansky, “Optoacoustic methods for frequency calibration of ultrasonic sensors,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control58(2), 316–326 (2011). [CrossRef] [PubMed]
  34. X. L. Deán-Ben, V. Ntziachristos, and D. Razansky, “Artefact reduction in optoacoustic tomographic imaging by estimating the distribution of acoustic scatterers,” J. Biomed. Opt.17(11), 110504 (2012). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4 Fig. 5

Supplementary Material

» Media 1: AVI (785 KB)     
» Media 2: MP4 (3445 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited