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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 23 — Nov. 18, 2013
  • pp: 28258–28271

Numerical refocusing in digital holographic microscopy with extended-sources illumination

Matěj Týč, Lukáš Kvasnica, Michala Slabá, and Radim Chmelík  »View Author Affiliations


Optics Express, Vol. 21, Issue 23, pp. 28258-28271 (2013)
http://dx.doi.org/10.1364/OE.21.028258


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Abstract

Numerical refocusing can be seen as a method of compensating the defocus aberration based on deconvolution by inverse filtering [1] in digital holographic microscopy (DHM). It is well-understood in cases when a coherent (ie point and monochromatic) light source such as a collimated laser beam is used [2]. This paper extends the theory to the case of illumination by a quasi-monochromatic extended (spatially incoherent) source. Refocusing methods for spatially incoherent illumination are derived and benefits of this type of illumination are demonstrated. We have proved both theoretically and experimentally that coherent-based refocusing gives incorrect results for extended-source illumination, while results obtained using the newly derived method are correct.

© 2013 Optical Society of America

OCIS Codes
(100.1830) Image processing : Deconvolution
(110.0180) Imaging systems : Microscopy
(110.4980) Imaging systems : Partial coherence in imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(090.1995) Holography : Digital holography

ToC Category:
Image Processing

History
Original Manuscript: August 13, 2013
Revised Manuscript: October 18, 2013
Manuscript Accepted: October 23, 2013
Published: November 11, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Matěj Týč, Lukáš Kvasnica, Michala Slabá, and Radim Chmelík, "Numerical refocusing in digital holographic microscopy with extended-sources illumination," Opt. Express 21, 28258-28271 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-23-28258


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