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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 23 — Nov. 18, 2013
  • pp: 28960–28967

Spectrally encoded angular light scattering

Jost Adam, Ata Mahjoubfar, Eric D. Diebold, Brandon W. Buckley, and Bahram Jalali  »View Author Affiliations


Optics Express, Vol. 21, Issue 23, pp. 28960-28967 (2013)
http://dx.doi.org/10.1364/OE.21.028960


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Abstract

The angular light scattering profile of microscopic particles significantly depends on their morphological parameters, such as size and shape. This dependency is widely used in state-of-the-art flow cytometry methods for particle classification. We introduce a new spectrally encoded angular light scattering method, with potential application in scanning flow cytometry. We show that a one-to-one wavelength-to-angle mapping enables the measurement of the angular dependence of scattered light from microscopic particles over a wide dynamic range. Improvement in dynamic range is obtained by equalizing the angular dependence of scattering via wavelength equalization. Continuous angular spectrum is obtained without mechanical scanning enabling single-shot measurement. Using this information, particle morphology can be determined with improved accuracy. We derive and experimentally verify an analytic wavelength-to-angle mapping model, facilitating rapid data processing. As a proof of concept, we demonstrate the method’s capability of distinguishing differently sized polystyrene beads. The combination of this technique with time-stretch dispersive Fourier transform offers real-time and high-throughput (high frame rate) measurements and renders the method suitable for integration in standard flow cytometers.

© 2013 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.7160) Medical optics and biotechnology : Ultrafast technology
(290.4020) Scattering : Mie theory
(290.5820) Scattering : Scattering measurements
(290.5850) Scattering : Scattering, particles

ToC Category:
Scattering

History
Original Manuscript: September 9, 2013
Revised Manuscript: October 14, 2013
Manuscript Accepted: October 16, 2013
Published: November 15, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Jost Adam, Ata Mahjoubfar, Eric D. Diebold, Brandon W. Buckley, and Bahram Jalali, "Spectrally encoded angular light scattering," Opt. Express 21, 28960-28967 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-23-28960


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