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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 24 — Dec. 2, 2013
  • pp: 29255–29268

Analysis of spectral excitation for measurements of fluorescence constituents in natural waters

Alexander Chekalyuk and Mark Hafez  »View Author Affiliations

Optics Express, Vol. 21, Issue 24, pp. 29255-29268 (2013)

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Field measurements of chlorophyll-a (Chl), phycoerythrin (PE), chromophoric dissolved organic matter (CDOM), and variable fluorescence (Fv/Fm) in diverse waters of the California Current, Mediterranean Sea and Gulf of Mexico using 375, 405, 510 and 532 nm laser excitation wavelengths (EW) are analyzed. EW = 375 and 405 nm were found more suitable for Chl assessment in high-Chl (> 10 μg/l) waters. Both EW = 532 and 510 nm can be used to efficiently stimulate PE fluorescence for structural characterization of phytoplankton communities. EW = 375 nm and 405 nm can provide best results for CDOM assessments in offshore oceanic waters; the green EWs can be also used for CDOM measurements in fresh and estuarine water types in conjunction with spectral discrimination between CDOM and PE fluorescence. Both EW = 405 and 510 are suitable for photo-physiological Fv/Fm assessments, though using EW = 405 nm may result in underestimation of PE-containing phytoplankton groups present in mixed phytoplankton assemblages.

© 2013 Optical Society of America

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(140.0140) Lasers and laser optics : Lasers and laser optics
(300.0300) Spectroscopy : Spectroscopy
(280.4788) Remote sensing and sensors : Optical sensing and sensors

ToC Category:
Atmospheric and Oceanic Optics

Original Manuscript: October 4, 2013
Revised Manuscript: November 4, 2013
Manuscript Accepted: November 5, 2013
Published: November 18, 2013

Virtual Issues
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics

Alexander Chekalyuk and Mark Hafez, "Analysis of spectral excitation for measurements of fluorescence constituents in natural waters," Opt. Express 21, 29255-29268 (2013)

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