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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 25 — Dec. 16, 2013
  • pp: 30874–30885

Towards in situ fluorescence spectroscopy and microscopy investigations of asphaltene precipitation kinetics

Juliana C. Franco, Grasiele Gonçalves, Monique S. Souza, Samantha B. C. Rosa, Larissa M. Thiegue, Teresa D. Z. Atvars, Paulo T. V. Rosa, and René A. Nome  »View Author Affiliations

Optics Express, Vol. 21, Issue 25, pp. 30874-30885 (2013)

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We perform a spectroscopic analysis of asphaltene in solution and in crude oil with the goal of designing an optical probe of asphaltene precipitation inside high-pressure cells. Quantitative analysis of steady-state spectroscopic data is employed to identify fluorescence and Raman contributions to the observed signals. Time-resolved fluorescence spectroscopy indicates that fluorescence lifetime can be used as a spectroscopic probe of asphaltene in crude oil. Quantitative confocal laser-scanning microscopy studies of asphaltene in n-heptane are used to calculate particle-size distributions as a function of time, both at the sample surface and asphaltene interior. The resulting precipitation kinetics is well described by stochastic numerical simulations of diffusion-limited aggregation. Based on these results, we present the design and construction of an apparatus to optically probe the in situ precipitation of asphaltene suitable for studies inside high pressure cells. Design considerations include the use of a spatial light modulator for aberration correction in microscopy measurements, together with the design of epi-fluorescence spectrometer, both fiber-based and for remote sensing fluorescence spectroscopy.

© 2013 Optical Society of America

OCIS Codes
(120.0280) Instrumentation, measurement, and metrology : Remote sensing and sensors
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:

Original Manuscript: October 11, 2013
Revised Manuscript: November 21, 2013
Manuscript Accepted: November 24, 2013
Published: December 6, 2013

Juliana C. Franco, Grasiele Gonçalves, Monique S. Souza, Samantha B. C. Rosa, Larissa M. Thiegue, Teresa D. Z. Atvars, Paulo T. V. Rosa, and René A. Nome, "Towards in situ fluorescence spectroscopy and microscopy investigations of asphaltene precipitation kinetics," Opt. Express 21, 30874-30885 (2013)

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