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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 25 — Dec. 16, 2013
  • pp: 31604–31614

Blu-ray disk lens as the objective of a miniaturized two-photon fluorescence microscope

Hsiang-Yu Chung, Wei-Cheng Kuo, Yu-Hsiang Cheng, Che-Hang Yu, Shih-Hsuan Chia, Cheng-Yung Lin, Jie-Shin Chen, Huai-Jen Tsai, Andrey B. Fedotov, Anatoly A. Ivanov, Aleksei M. Zheltikov, and Chi-Kuang Sun  »View Author Affiliations


Optics Express, Vol. 21, Issue 25, pp. 31604-31614 (2013)
http://dx.doi.org/10.1364/OE.21.031604


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Abstract

In this paper, we examine the performance of a Blu-ray disk (BD) aspheric lens as the objective of a miniaturized scanning nonlinear optical microscope. By combining a single 2D micro-electro mechanical system (MEMS) mirror as the scanner and with different tube lens pairs, the field of view (FOV) of the studied microscope varies from 59 μm × 93 μm up to 178 μm × 280 μm, while the corresponding lateral resolution varies from 0.6 μm to 2 μm for two-photon fluorescence (2PF) signals. With a 34/s video frame rate, in vivo dynamic observation of zebrafish heartbeat through 2PF of the excited green fluorescence protein (GFP) is demonstrated.

© 2013 Optical Society of America

OCIS Codes
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials
(220.4830) Optical design and fabrication : Systems design
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: August 30, 2013
Revised Manuscript: November 1, 2013
Manuscript Accepted: November 2, 2013
Published: December 13, 2013

Virtual Issues
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Hsiang-Yu Chung, Wei-Cheng Kuo, Yu-Hsiang Cheng, Che-Hang Yu, Shih-Hsuan Chia, Cheng-Yung Lin, Jie-Shin Chen, Huai-Jen Tsai, Andrey B. Fedotov, Anatoly A. Ivanov, Aleksei M. Zheltikov, and Chi-Kuang Sun, "Blu-ray disk lens as the objective of a miniaturized two-photon fluorescence microscope," Opt. Express 21, 31604-31614 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-25-31604


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