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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 26 — Dec. 30, 2013
  • pp: 31782–31788

Total liquid ventilation: a new approach to improve 3D OCT image quality of alveolar structures in lung tissue

Christian Schnabel, Maria Gaertner, Lars Kirsten, Sven Meissner, and Edmund Koch  »View Author Affiliations

Optics Express, Vol. 21, Issue 26, pp. 31782-31788 (2013)

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Little is known about mechanical processes of alveolar tissue during mechanical ventilation. Optical coherence tomography (OCT) as a three-dimensional and high-resolution imaging modality can be used to visualize subpleural alveoli during artificial ventilation. The quality of OCT images can be increased by matching the refractive index inside the alveoli to the one of tissue via liquid-filling. Thereby, scattering loss can be decreased and higher penetration depth and tissue contrast can be achieved. We show the liquid-filling of alveolar structures verified by optical coherence tomography and intravital microscopy (IVM) and the advantages of index matching for OCT imaging of subpleural alveoli in a mouse model using a custom-made liquid ventilator.

© 2013 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(170.1650) Medical optics and biotechnology : Coherence imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 17, 2013
Revised Manuscript: November 16, 2013
Manuscript Accepted: November 18, 2013
Published: December 16, 2013

Virtual Issues
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics

Christian Schnabel, Maria Gaertner, Lars Kirsten, Sven Meissner, and Edmund Koch, "Total liquid ventilation: a new approach to improve 3D OCT image quality of alveolar structures in lung tissue," Opt. Express 21, 31782-31788 (2013)

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