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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 4 — Feb. 25, 2013
  • pp: 5164–5170

Fluorescence imaging of flowing cells using a temporally coded excitation

Sai Siva Gorthi, Diane Schaak, and Ethan Schonbrun  »View Author Affiliations

Optics Express, Vol. 21, Issue 4, pp. 5164-5170 (2013)

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Imaging fluorescence in moving cells is fundamentally challenging because the exposure time is constrained by motion-blur, which limits the available signal. We report a method to image fluorescently labeled leukemia cells in fluid flow that has an effective exposure time of up to 50 times the motion-blur limit. Flowing cells are illuminated with a pseudo-random excitation pulse sequence, resulting in a motion-blur that can be computationally removed to produce near diffraction-limited images. This method enables observation of cellular organelles and their behavior in a fluid environment that resembles the vasculature.

© 2013 OSA

OCIS Codes
(100.1830) Image processing : Deconvolution
(110.4280) Imaging systems : Noise in imaging systems
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:

Original Manuscript: December 13, 2012
Revised Manuscript: February 1, 2013
Manuscript Accepted: February 14, 2013
Published: February 22, 2013

Virtual Issues
Vol. 8, Iss. 3 Virtual Journal for Biomedical Optics

Sai Siva Gorthi, Diane Schaak, and Ethan Schonbrun, "Fluorescence imaging of flowing cells using a temporally coded excitation," Opt. Express 21, 5164-5170 (2013)

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