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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 6 — Mar. 25, 2013
  • pp: 7362–7372

Differential interferometric particle tracking on the subnanometer- and submillisecond-scale

Dennis Müller, Dieter R. Klopfenstein, and Rainer G. Ulbrich  »View Author Affiliations

Optics Express, Vol. 21, Issue 6, pp. 7362-7372 (2013)

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We describe an interferometric method to measure the movement of a subwavelength probe particle relative to an immobilized reference particle with high spatial (Δx = 0.9nm) and temporal (Δt = 200μs) resolution. The differential method eliminates microscope stage drift. An upright microscope is equipped with laser dark field illumination (λ0 = 532nm, P0 = 30mW) and a compact modified Mach-Zehnder interferometer is mounted on the camera exit of the microscope, where the beams of scattered light of both particles are combined. The resulting interferograms provide in two channels subnanometer information about the motion of the probe particle relative to the reference particle. The interferograms are probed with two avalanche photodiodes. We applied this method to measuring the movement of kinesin along microtubules and were able to resolve the generic 8-nm steps at high ATP concentrations without external forces.

© 2013 OSA

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.3170) Microscopy : Interference microscopy

ToC Category:

Original Manuscript: December 19, 2012
Revised Manuscript: February 15, 2013
Manuscript Accepted: February 21, 2013
Published: March 18, 2013

Virtual Issues
Vol. 8, Iss. 4 Virtual Journal for Biomedical Optics

Dennis Müller, Dieter R. Klopfenstein, and Rainer G. Ulbrich, "Differential interferometric particle tracking on the subnanometer- and submillisecond-scale," Opt. Express 21, 7362-7372 (2013)

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