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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 7 — Apr. 8, 2013
  • pp: 8793–8798

Dye exclusion microfluidic microscopy

Ethan Schonbrun, Giuseppe Di Caprio, and Diane Schaak  »View Author Affiliations

Optics Express, Vol. 21, Issue 7, pp. 8793-8798 (2013)

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We present an optical system to measure height maps of non-adherent cells as they flow through a microfluidic channel. The cells are suspended in an index-matching absorbing buffer, where cell height is evaluated by measuring the difference in absorption between the cell and the background. Unlike interferometric microscopes, the measured cell height is nearly independent of the cell’s optical properties. The height maps are captured using a single exposure of a color camera, and consequently the system is capable of high-throughput characterization of large collections of cells. Using this system, we have measured more than 1600 height maps and volumes of three different leukemia cell lines.

© 2013 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: February 19, 2013
Revised Manuscript: March 20, 2013
Manuscript Accepted: March 26, 2013
Published: April 2, 2013

Virtual Issues
Vol. 8, Iss. 5 Virtual Journal for Biomedical Optics

Ethan Schonbrun, Giuseppe Di Caprio, and Diane Schaak, "Dye exclusion microfluidic microscopy," Opt. Express 21, 8793-8798 (2013)

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