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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 8 — Apr. 22, 2013
  • pp: 9839–9850

Visualization of brain circuits using two-photon fluorescence micro-optical sectioning tomography

Ting Zheng, Zhongqing Yang, Anan Li, Xiaohua Lv, Zhenqiao Zhou, Xiaojun Wang, Xiaoli Qi, Shiwei Li, Qingming Luo, Hui Gong, and Shaoqun Zeng  »View Author Affiliations

Optics Express, Vol. 21, Issue 8, pp. 9839-9850 (2013)

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Neural circuits are fundamental for brain functions. However, obtaining long range continuous projections of neurons in the entire brain is still challenging. Here a two-photon fluorescence micro-optical sectioning tomography (2p-fMOST) method is developed for high-throughput, high-resolution visualization of the brain circuits. Two-photon imaging technology is used to obtain high resolution, and acoustical optical deflector (AOD), an inertia-free beam scanner is used to realize fast and prolonged stable imaging. The combination of these techniques with imaging and then sectioning method of a plastic-embedded mouse brain facilitated the acquisition of a three-dimensional data set of a fluorescent mouse brain with a resolution adequate to resolve the spines. In addition, the brain circuit tracing ability is showed by several neurons projecting across different brain regions. Besides brain imaging, 2p-fMOST could be used in many studies that requires sub-micro resolution or micro resolution imaging of a large sample.

© 2013 OSA

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(230.1040) Optical devices : Acousto-optical devices
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 15, 2013
Revised Manuscript: March 28, 2013
Manuscript Accepted: March 31, 2013
Published: April 12, 2013

Virtual Issues
Vol. 8, Iss. 5 Virtual Journal for Biomedical Optics

Ting Zheng, Zhongqing Yang, Anan Li, Xiaohua Lv, Zhenqiao Zhou, Xiaojun Wang, Xiaoli Qi, Shiwei Li, Qingming Luo, Hui Gong, and Shaoqun Zeng, "Visualization of brain circuits using two-photon fluorescence micro-optical sectioning tomography," Opt. Express 21, 9839-9850 (2013)

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