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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 10 — May. 19, 2014
  • pp: 12102–12114

Recovery of Raman spectra with low signal-to-noise ratio using Wiener estimation

Shuo Chen, Xiaoqian Lin, Clement Yuen, Saraswathi Padmanabhan, Roger W. Beuerman, and Quan Liu  »View Author Affiliations

Optics Express, Vol. 22, Issue 10, pp. 12102-12114 (2014)

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Raman spectroscopy is a powerful non-destructive technique for qualitatively and quantitatively characterizing materials. However, noise often obscures interesting Raman peaks due to the inherently weak Raman signal, especially in biological samples. In this study, we develop a method based on spectral reconstruction to recover Raman spectra with low signal-to-noise ratio (SNR). The synthesis of narrow-band measurements from low-SNR Raman spectra eliminates the effect of noise by integrating the Raman signal along the wavenumber dimension, which is followed by spectral reconstruction based on Wiener estimation to recover the Raman spectrum with high spectral resolution. Non-negative principal components based filters are used in the synthesis to ensure that most variance contained in the original Raman measurements are retained. A total of 25 agar phantoms and 20 bacteria samples were measured and data were used to validate our method. Four commonly used de-noising methods in Raman spectroscopy, i.e. Savitzky-Golay (SG) algorithm, finite impulse response (FIR) filtration, wavelet transform and factor analysis, were also evaluated on the same set of data in addition to the proposed method for comparison. The proposed method showed the superior accuracy in the recovery of Raman spectra from measurements with extremely low SNR, compared with the four commonly used de-noising methods.

© 2014 Optical Society of America

OCIS Codes
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(300.6170) Spectroscopy : Spectra
(300.6450) Spectroscopy : Spectroscopy, Raman

ToC Category:

Original Manuscript: March 24, 2014
Revised Manuscript: May 3, 2014
Manuscript Accepted: May 4, 2014
Published: May 12, 2014

Virtual Issues
Vol. 9, Iss. 7 Virtual Journal for Biomedical Optics

Shuo Chen, Xiaoqian Lin, Clement Yuen, Saraswathi Padmanabhan, Roger W. Beuerman, and Quan Liu, "Recovery of Raman spectra with low signal-to-noise ratio using Wiener estimation," Opt. Express 22, 12102-12114 (2014)

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