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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 10 — May. 19, 2014
  • pp: 12255–12272

Estimation of the number of fluorescent end-members for quantitative analysis of multispectral FLIM data

Omar Gutierrez-Navarro, Daniel U. Campos-Delgado, Edgar R. Arce-Santana, Kristen C. Maitland, Shuna Cheng, Joey Jabbour, Bilal Malik, Rodrigo Cuenca, and Javier A. Jo  »View Author Affiliations


Optics Express, Vol. 22, Issue 10, pp. 12255-12272 (2014)
http://dx.doi.org/10.1364/OE.22.012255


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Abstract

Multispectral fluorescence lifetime imaging (m-FLIM) can potentially allow identifying the endogenous fluorophores present in biological tissue. Quantitative description of such data requires estimating the number of components in the sample, their characteristic fluorescent decays, and their relative contributions or abundances. Unfortunately, this inverse problem usually requires prior knowledge about the data, which is seldom available in biomedical applications. This work presents a new methodology to estimate the number of potential endogenous fluorophores present in biological tissue samples from time-domain m-FLIM data. Furthermore, a completely blind linear unmixing algorithm is proposed. The method was validated using both synthetic and experimental m-FLIM data. The experimental m-FLIM data include in-vivo measurements from healthy and cancerous hamster cheek-pouch epithelial tissue, and ex-vivo measurements from human coronary atherosclerotic plaques. The analysis of m-FLIM data from in-vivo hamster oral mucosa identified healthy from precancerous lesions, based on the relative concentration of their characteristic fluorophores. The algorithm also provided a better description of atherosclerotic plaques in term of their endogenous fluorophores. These results demonstrate the potential of this methodology to provide quantitative description of tissue biochemical composition.

© 2014 Optical Society of America

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.6920) Medical optics and biotechnology : Time-resolved imaging

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: March 17, 2014
Revised Manuscript: May 2, 2014
Manuscript Accepted: May 2, 2014
Published: May 13, 2014

Virtual Issues
Vol. 9, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Omar Gutierrez-Navarro, Daniel U. Campos-Delgado, Edgar R. Arce-Santana, Kristen C. Maitland, Shuna Cheng, Joey Jabbour, Bilal Malik, Rodrigo Cuenca, and Javier A. Jo, "Estimation of the number of fluorescent end-members for quantitative analysis of multispectral FLIM data," Opt. Express 22, 12255-12272 (2014)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-22-10-12255


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