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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 10 — May. 19, 2014
  • pp: 12513–12523

Ptychographic microscope for three-dimensional imaging

T. M. Godden, R. Suman, M. J. Humphry, J. M. Rodenburg, and A. M. Maiden  »View Author Affiliations

Optics Express, Vol. 22, Issue 10, pp. 12513-12523 (2014)

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Ptychography is a coherent imaging technique that enables an image of a specimen to be generated from a set of diffraction patterns. One limitation of the technique is the assumption of a multiplicative interaction between the illuminating coherent beam and the specimen, which restricts ptychography to samples no thicker than a few tens of micrometers in the case of visible-light imaging at micron-scale resolution. By splitting a sample into axial sections, we demonstrated in recent work that this thickness restriction can be relaxed and whats-more, that coarse optical sectioning can be realized using a single ptychographic data set. Here we apply our technique to data collected from a modified optical microscope to realize a reduction in the optical sectioning depth to 2 μm in the axial direction for samples up to 150 μm thick. Furthermore, we increase the number of sections that are imaged from 5 in our previous work to 34 here. Our results compare well with sectioned images collected from a confocal microscope but have the added advantage of strong phase contrast, which removes the need for sample staining.

© 2014 Optical Society of America

OCIS Codes
(100.3190) Image processing : Inverse problems
(100.5070) Image processing : Phase retrieval
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: April 1, 2014
Revised Manuscript: May 6, 2014
Manuscript Accepted: May 7, 2014
Published: May 15, 2014

Virtual Issues
Vol. 9, Iss. 7 Virtual Journal for Biomedical Optics

T. M. Godden, R. Suman, M. J. Humphry, J. M. Rodenburg, and A. M. Maiden, "Ptychographic microscope for three-dimensional imaging," Opt. Express 22, 12513-12523 (2014)

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