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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 11 — Jun. 2, 2014
  • pp: 12962–12970

Gradient index lens based combined two-photon microscopy and optical coherence tomography

Taejun Wang, Qingyun Li, Peng Xiao, Jinhyo Ahn, Young Eun Kim, Youngrong Park, Minjun Kim, Miyeoun Song, Euiheon Chung, Wan Kyun Chung, G-One Ahn, Sungjee Kim, Pilhan Kim, Seung-Jae Myung, and Ki Hean Kim  »View Author Affiliations

Optics Express, Vol. 22, Issue 11, pp. 12962-12970 (2014)

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We report a miniaturized probe-based combined two-photon microscopy (TPM) and optical coherence tomography (OCT) system. This system is to study the colorectal cancer in mouse models by visualizing both cellular and structural information of the colon in 3D with TPM and OCT respectively. The probe consisted of gradient index (GRIN) lenses and a 90° reflecting prism at its distal end for side-viewing, and it was added onto an objective lens-based TPM and OCT system. The probe was 2.2 mm in diameter and 60 mm in length. TPM imaging was performed by raster scanning of the excitation focus at the imaging speed of 15.4 frames/s. OCT imaging was performed by combining the linear sample translation and probe rotation along its axis. This miniaturized probe based dual-modal system was characterized with tissue phantoms containing fluorescent microspheres, and applied to image mouse colonic tissues ex vivo as a demonstration. As OCT and TPM provided structural and cellular information of the tissues respectively, this probe based multi-modal imaging system can be helpful for in vivo studies of preclinical animal models such as mouse colonic tumorigenesis.

© 2014 Optical Society of America

OCIS Codes
(110.2760) Imaging systems : Gradient-index lenses
(110.4500) Imaging systems : Optical coherence tomography
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2680) Medical optics and biotechnology : Gastrointestinal
(180.2520) Microscopy : Fluorescence microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 14, 2014
Revised Manuscript: April 24, 2014
Manuscript Accepted: April 24, 2014
Published: May 21, 2014

Virtual Issues
Vol. 9, Iss. 8 Virtual Journal for Biomedical Optics

Taejun Wang, Qingyun Li, Peng Xiao, Jinhyo Ahn, Young Eun Kim, Youngrong Park, Minjun Kim, Miyeoun Song, Euiheon Chung, Wan Kyun Chung, G-One Ahn, Sungjee Kim, Pilhan Kim, Seung-Jae Myung, and Ki Hean Kim, "Gradient index lens based combined two-photon microscopy and optical coherence tomography," Opt. Express 22, 12962-12970 (2014)

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