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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 11 — Jun. 2, 2014
  • pp: 13073–13090

Scanning inverse fluorescence correlation spectroscopy

Jan Bergstrand, Daniel Rönnlund, Jerker Widengren, and Stefan Wennmalm  »View Author Affiliations

Optics Express, Vol. 22, Issue 11, pp. 13073-13090 (2014)

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Scanning Inverse Fluorescence Correlation Spectroscopy (siFCS) is introduced to determine the absolute size of nanodomains on surfaces. We describe here equations for obtaining the domain size from cross- and auto-correlation functions, measurement simulations which enabled testing of these equations, and measurements on model surfaces mimicking membranes containing nanodomains. Using a confocal microscope of 270 nm resolution the size of 250 nm domains were estimated by siFCS to 257 ± 12 nm diameter, and 40 nm domains were estimated to 65 ± 26 nm diameter. Applications of siFCS for sizing of nanodomains and protein clusters in cell membranes are discussed.

© 2014 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:

Original Manuscript: February 26, 2014
Revised Manuscript: April 1, 2014
Manuscript Accepted: April 2, 2014
Published: May 22, 2014

Virtual Issues
Vol. 9, Iss. 8 Virtual Journal for Biomedical Optics

Jan Bergstrand, Daniel Rönnlund, Jerker Widengren, and Stefan Wennmalm, "Scanning inverse fluorescence correlation spectroscopy," Opt. Express 22, 13073-13090 (2014)

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