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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 11 — Jun. 2, 2014
  • pp: 13472–13483

Rapid quantitative phase imaging for partially coherent light microscopy

José A. Rodrigo and Tatiana Alieva  »View Author Affiliations


Optics Express, Vol. 22, Issue 11, pp. 13472-13483 (2014)
http://dx.doi.org/10.1364/OE.22.013472


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Abstract

Partially coherent light provides promising advantages for imaging applications. In contrast to its completely coherent counterpart, it prevents image degradation due to speckle noise and decreases cross-talk among the imaged objects. These facts make attractive the partially coherent illumination for accurate quantitative imaging in microscopy. In this work, we present a non-interferometric technique and system for quantitative phase imaging with simultaneous determination of the spatial coherence properties of the sample illumination. Its performance is experimentally demonstrated in several examples underlining the benefits of partial coherence for practical imagining applications. The programmable optical setup comprises an electrically tunable lens and sCMOS camera that allows for high-speed measurement in the millisecond range.

© 2014 Optical Society of America

OCIS Codes
(030.0030) Coherence and statistical optics : Coherence and statistical optics
(100.3010) Image processing : Image reconstruction techniques
(100.5070) Image processing : Phase retrieval
(110.0180) Imaging systems : Microscopy
(120.4630) Instrumentation, measurement, and metrology : Optical inspection
(120.5050) Instrumentation, measurement, and metrology : Phase measurement

ToC Category:
Microscopy

History
Original Manuscript: March 28, 2014
Revised Manuscript: May 12, 2014
Manuscript Accepted: May 16, 2014
Published: May 27, 2014

Virtual Issues
Vol. 9, Iss. 8 Virtual Journal for Biomedical Optics

Citation
José A. Rodrigo and Tatiana Alieva, "Rapid quantitative phase imaging for partially coherent light microscopy," Opt. Express 22, 13472-13483 (2014)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-22-11-13472


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