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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 11 — Jun. 2, 2014
  • pp: 13733–13743

RP-CARS: label-free optical readout of the myelin intrinsic healthiness

Giuseppe de Vito, Ilaria Tonazzini, Marco Cecchini, and Vincenzo Piazza  »View Author Affiliations


Optics Express, Vol. 22, Issue 11, pp. 13733-13743 (2014)
http://dx.doi.org/10.1364/OE.22.013733


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Abstract

Here we present a method based on Rotating-Polarization Coherent Anti-Stokes Raman Scattering (RP-CARS) imaging to assess the myelin health status in mouse sciatic nerves. Differently from the existing techniques, our method is based on the readout of intrinsic molecular architecture rather than on the image analysis, relying on the fact that healthy myelin is characterized by a high degree of molecular order. We exploit RP-CARS imaging to demonstrate that the degree of spatial anisotropy of the CARS signal displays a strong correlation with the g-ratio (a well-known image-based index of myelin damage) in a chemical-damage model and therefore that the former is a good indicator for the local myelin health status.

© 2014 Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4730) Medical optics and biotechnology : Optical pathology
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 12, 2014
Revised Manuscript: April 19, 2014
Manuscript Accepted: May 18, 2014
Published: May 30, 2014

Citation
Giuseppe de Vito, Ilaria Tonazzini, Marco Cecchini, and Vincenzo Piazza, "RP-CARS: label-free optical readout of the myelin intrinsic healthiness," Opt. Express 22, 13733-13743 (2014)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-22-11-13733


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