OSA's Digital Library

Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 14 — Jul. 14, 2014
  • pp: 17063–17072

Photoacoustic microscopy using ultrashort pulses with two different pulse durations

Yoshihisa Yamaoka, Yoshinori Harada, Masaaki Sakakura, Takeo Minamikawa, Shigeru Nishino, Seiji Maehara, Shujiro Hamano, Hideo Tanaka, and Tetsuro Takamatsu  »View Author Affiliations

Optics Express, Vol. 22, Issue 14, pp. 17063-17072 (2014)

View Full Text Article

Enhanced HTML    Acrobat PDF (2403 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We propose photoacoustic microscopy using ultrashort pulses with two different pulse durations in the range from femtoseconds to picoseconds. The subtraction of images for longer-pulse excitation from those for shorter-pulse excitation extracts two-photon photoacoustic images effectively, based on observation that the intensity ratio of two-photon to one-photon absorption-induced photoacoustic signals depends on the pulse duration in the same manner as the intensity ratio of two-photon and one-photon fluorescence signals. Two-photon photoacoustic microscopy using this subtraction method enables precise observation of the cross-sections of silicone hollows filled with the mixture of one-photon and two-photon absorption solutions.

© 2014 Optical Society of America

OCIS Codes
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(320.7090) Ultrafast optics : Ultrafast lasers
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: May 7, 2014
Revised Manuscript: June 19, 2014
Manuscript Accepted: June 20, 2014
Published: July 3, 2014

Virtual Issues
Vol. 9, Iss. 9 Virtual Journal for Biomedical Optics

Yoshihisa Yamaoka, Yoshinori Harada, Masaaki Sakakura, Takeo Minamikawa, Shigeru Nishino, Seiji Maehara, Shujiro Hamano, Hideo Tanaka, and Tetsuro Takamatsu, "Photoacoustic microscopy using ultrashort pulses with two different pulse durations," Opt. Express 22, 17063-17072 (2014)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. Y. Yamaoka, M. Nambu, and T. Takamatsu, “Fine depth resolution of two-photon absorption-induced photoacoustic microscopy using low-frequency bandpass filtering,” Opt. Express 19(14), 13365–13377 (2011). [CrossRef] [PubMed]
  2. Y. Yamaoka, Y. Harada, S. Nishino, S. Maehara, S. Hamano, and T. Takamatsu, “Improvement of signal detection selectivity and efficiency in two-photon absorption-induced photoacoustic microscopy,” Proc. SPIE 8943, 89433C (2014). [CrossRef]
  3. Y. H. Lai, S. Y. Lee, C. F. Chang, Y. H. Cheng, and C. K. Sun, “Nonlinear photoacoustic microscopy via a loss modulation technique: from detection to imaging,” Opt. Express 22(1), 525–536 (2014). [CrossRef] [PubMed]
  4. G. Langer, K. D. Bouchal, H. Grün, P. Burgholzer, and T. Berer, “Two-photon absorption-induced photoacoustic imaging of Rhodamine B dyed polyethylene spheres using a femtosecond laser,” Opt. Express 21(19), 22410–22422 (2013). [CrossRef] [PubMed]
  5. Y. Yamaoka, M. Nambu, and T. Takamatsu, “Frequency-selective multiphoton-excitation-induced photoacoustic microscopy (MEPAM) to visualize the cross sections of dense objects,” Proc. SPIE 7564, 75642O (2010). [CrossRef]
  6. M. E. van Raaij, M. Lee, E. Chérin, B. Stefanovic, and F. S. Foster, “Femtosecond photoacoustics: integrated two-photon fluorescence and photoacoustic microscopy,” Proc. SPIE 7564, 75642E (2010). [CrossRef]
  7. Y. Yamaoka and T. Takamatsu, “Enhancement of multiphoton excitation-induced photoacoustic signals by using gold nanoparticles surrounded by fluorescent dyes,” Proc. SPIE 7177, 71772A (2009). [CrossRef]
  8. Y. Yamaoka, K. Fujiwara, and T. Takamatsu, “Improvement of depth resolution on photoacoustic imaging using multiphoton absorption,” Proc. SPIE 6631, 663102 (2007). [CrossRef]
  9. P. C. Li, C. W. Wei, and Y. L. Sheu, “Subband photoacoustic imaging for contrast improvement,” Opt. Express 16(25), 20215–20226 (2008). [CrossRef] [PubMed]
  10. P. Tian and W. S. Warren, “Ultrafast measurement of two-photon absorption by loss modulation,” Opt. Lett. 27(18), 1634–1636 (2002). [CrossRef] [PubMed]
  11. J. Yao, H. Ke, S. Tai, Y. Zhou, and L. V. Wang, “Absolute photoacoustic thermometry in deep tissue,” Opt. Lett. 38(24), 5228–5231 (2013). [CrossRef] [PubMed]
  12. J. Kandulla, H. Elsner, R. Birngruber, and R. Brinkmann, “Noninvasive optoacoustic online retinal temperature determination during continuous-wave laser irradiation,” J. Biomed. Opt. 11(4), 041111 (2006). [CrossRef] [PubMed]
  13. J. M. Khosrofian and B. A. Garetz, “Measurement of a Gaussian laser beam diameter through the direct inversion of knife-edge data,” Appl. Opt. 22(21), 3406–3410 (1983). [CrossRef] [PubMed]
  14. C. Xu and W. W. Webb, “Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm,” J. Opt. Soc. Am. B 13(3), 481–491 (1996). [CrossRef]
  15. M. Rumi and J. W. Perry, “Two-photon absorption: an overview of measurements and principles,” Adv. Opt. Photon. 2(4), 451–518 (2010). [CrossRef]
  16. R. W. Boyd, Nonlinear Optics (Academic, 1992).
  17. G. P. Agrawal, Nonlinear Fiber Optics, 2nd ed. (Academic, 1995).
  18. V. E. Gusev and A. A. Karabutov, Laser Optoacoustics (American Institute of Physics, 1993).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited