OSA's Digital Library

Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 15 — Jul. 28, 2014
  • pp: 17830–17839

Quantitative phase-contrast confocal microscope

Changgeng Liu, Stefano Marchesini, and Myung K. Kim  »View Author Affiliations


Optics Express, Vol. 22, Issue 15, pp. 17830-17839 (2014)
http://dx.doi.org/10.1364/OE.22.017830


View Full Text Article

Enhanced HTML    Acrobat PDF (5826 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present a quantitative phase-contrast confocal microscope (QPCCM) by combining a line-scanning confocal system with digital holography (DH). This combination can merge the merits of these two different imaging modalities. High-contrast intensity images with low coherent noise, and the optical sectioning capability are made available due to the confocality. Phase profiles of the samples become accessible thanks to DH. QPCCM is able to quantitatively measure the phase variations of optical sections of the opaque samples and has the potential to take high-quality intensity and phase images of non-opaque samples such as many biological samples. Because each line scan is recorded by a hologram that may contain the optical aberrations of the system, it opens avenues for a variety of numerical aberration compensation methods and development of full digital adaptive optics confocal system to emulate current hardware-based adaptive optics system for biomedical imaging, especially ophthalmic imaging. Preliminary experiments with a microscope objective of NA 0.65 and 40 × on opaque samples are presented to demonstrate this idea. The measured lateral and axial resolutions of the intensity images from the current system are ~0.64μm and ~2.70μm respectively. The noise level of the phase profile by QPCCM is ~2.4nm which is better than the result by DH.

© 2014 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: May 27, 2014
Revised Manuscript: July 6, 2014
Manuscript Accepted: July 7, 2014
Published: July 15, 2014

Virtual Issues
Vol. 9, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Changgeng Liu, Stefano Marchesini, and Myung K. Kim, "Quantitative phase-contrast confocal microscope," Opt. Express 22, 17830-17839 (2014)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-22-15-17830


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. M. Minsky, “Microscopy apparatus,” U.S. patent 3,013,467 (December 1961).
  2. R. H. Webb, “Confocal optical microscopy,” Rep. Prog. Phys.59(3), 427–471 (1996). [CrossRef]
  3. J. B. Pawley, ed., Handbook of Biological Confocal Microscopy (Springer, 1995).
  4. T. Wilson, ed., Confocal Microscopy (Academic, 1990).
  5. T. R. Corle and G. S. Kino, eds., Confocal Scanning Optical Microscopy and Related Imaging Systems (Academic, 1996).
  6. K. B. Im, S. Han, H. Park, D. Kim, and B. M. Kim, “Simple high-speed confocal line-scanning microscope,” Opt. Express13(13), 5151–5156 (2005). [CrossRef] [PubMed]
  7. P. J. Dwyer, C. A. DiMarzio, J. M. Zavislan, W. J. Fox, and M. Rajadhyaksha, “Confocal reflectance theta line scanning microscope for imaging human skin in vivo,” Opt. Lett.31(7), 942–944 (2006). [CrossRef] [PubMed]
  8. D. X. Hammer, R. D. Ferguson, T. E. Ustun, C. E. Bigelow, N. V. Iftimia, and R. H. Webb, “Line-scanning laser ophthalmoscope,” J. Biomed. Opt.11(4), 041126 (2006). [CrossRef] [PubMed]
  9. P. J. Dwyer, C. A. DiMarzio, and M. Rajadhyaksha, “Confocal theta line-scanning microscope for imaging human tissues,” Appl. Opt.46(10), 1843–1851 (2007). [CrossRef] [PubMed]
  10. M. Mujat, R. D. Ferguson, N. Iftimia, and D. X. Hammer, “Compact adaptive optics line scanning ophthalmoscope,” Opt. Express17(12), 10242–10258 (2009). [CrossRef] [PubMed]
  11. E. Cuche, F. Bevilacqua, and C. Depeursinge, “Digital holography for quantitative phase-contrast imaging,” Opt. Lett.24(5), 291–293 (1999). [CrossRef] [PubMed]
  12. C. Mann, L. Yu, C. M. Lo, and M. K. Kim, “High-resolution quantitative phase-contrast microscopy by digital holography,” Opt. Express13(22), 8693–8698 (2005). [CrossRef] [PubMed]
  13. M. K. Kim, “Principles and techniques of digital holographic microscopy,” SPIE Reviews1, 1–50 (2010).
  14. C. Liu and M. K. Kim, “Digital holographic adaptive optics for ocular imaging: proof of principle,” Opt. Lett.36(14), 2710–2712 (2011). [CrossRef] [PubMed]
  15. C. Liu, X. Yu, and M. K. Kim, “Fourier transform digital holographic adaptive optics imaging system,” Appl. Opt.51(35), 8449–8454 (2012). [CrossRef] [PubMed]
  16. A. S. Goy and D. Psaltis, “Digital confocal microscope,” Opt. Express20(20), 22720–22727 (2012). [CrossRef] [PubMed]
  17. A. S. Goy, M. Unser, and D. Psaltis, “Multiple contrast metrics from the measurements of a digital confocal microscope,” Biomed. Opt. Express4(7), 1091–1103 (2013). [CrossRef] [PubMed]
  18. J. R. Fienup and J. J. Miller, “Aberration correction by maximizing generalized sharpness metrics,” J. Opt. Soc. Am. A20(4), 609–620 (2003). [CrossRef] [PubMed]
  19. S. T. Thurman and J. R. Fienup, “Phase-error correction in digital holography,” J. Opt. Soc. Am. A25(4), 983–994 (2008). [CrossRef] [PubMed]
  20. T. Wilson and A. R. Carlini, “Size of the detector in confocal imaging systems,” Opt. Lett.12(4), 227–229 (1987). [CrossRef] [PubMed]
  21. A. Khmaladze, M. K. Kim, and C. M. Lo, “Phase imaging of cells by simultaneous dual-wavelength reflection digital holography,” Opt. Express16(15), 10900–10911 (2008). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Supplementary Material


» Media 1: AVI (131089 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited