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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 15 — Jul. 28, 2014
  • pp: 18101–18112

High throughput multichannel fluorescence microscopy with microlens arrays

Antony Orth and Kenneth B. Crozier  »View Author Affiliations


Optics Express, Vol. 22, Issue 15, pp. 18101-18112 (2014)
http://dx.doi.org/10.1364/OE.22.018101


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Abstract

We present a multichannel fluorescence microscopy technique for high throughput imaging applications. A microlens array with over 140,000 elements is used to image centimeter-scale samples at up to 18.1 megapixels per second. Large field-of-view multichannel fluorescent imaging is demonstrated in both sequential and parallel geometries. The extended dynamic range of this approach is also discussed.

© 2014 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.5810) Medical optics and biotechnology : Scanning microscopy

ToC Category:
Microscopy

History
Original Manuscript: May 19, 2014
Revised Manuscript: July 7, 2014
Manuscript Accepted: July 7, 2014
Published: July 18, 2014

Virtual Issues
Vol. 9, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Antony Orth and Kenneth B. Crozier, "High throughput multichannel fluorescence microscopy with microlens arrays," Opt. Express 22, 18101-18112 (2014)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-22-15-18101


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